Nguyen Nam V, Linderman Susanne L, Fashina Tolulope, Devine Max, Conrady Christopher D, Shantha Jessica G, Ahmed Rafi, Yeh Steven
Department of Ophthalmology, University of Nebraska Medical Center, Omaha, NE, USA.
College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA.
Int Med Case Rep J. 2024 Dec 19;17:1055-1061. doi: 10.2147/IMCRJ.S496941. eCollection 2024.
To report cytokine/chemokine profiles of ocular fluid in two patients with herpetic uveitis.
Cytokine and chemokine profiling of ocular fluid was performed in two patients with herpetic uveitis. Ocular fluid findings were correlated with disease manifestations and the patients' clinical course.
Case 1 was a 45-year-old female, who was evaluated for an 11-day history of recurrent redness, and decreased vision in the right eye (OD) and was diagnosed with acute retinal necrosis. Ocular fluid from anterior chamber paracentesis was positive for varicella zoster virus (VZV) via PCR testing. Subsequently, the patient developed proliferative vitreoretinopathy requiring a pars plana vitrectomy. Ocular fluid sample cytokine/chemokine analysis detected IFN-γ, TNF-α, IL-8, IL-18, MIP-1β, IP-10, and MCP-1 with MCP-1 being the most abundant cytokine. Case 2 was a 30-year-old female with a two-month history of progressive pain and decreased vision OD. She was diagnosed with hypertensive anterior uveitis after diagnostic anterior chamber paracentesis. Despite successful therapy for the anterior uveitis, her intraocular pressure remained elevated and required a glaucoma filtration procedure. Ocular fluid sample was collected at the time of surgery for cytokine/chemokine profiles analysis, and levels of 7 cytokines/chemokines were detected including IL-6, IL-8, IL-18, MIP-1β, IP-10, MCP-1, and IL-1RA with IL-1RA being the most abundant cytokine.
Cytokine/chemokine profiles of two patients with herpetic uveitis showed elevated levels of MCP-1, IP-10, IL-8, and IL-18 while IL-1RA was elevated in the chronic phase of hypertensive anterior uveitis. Further studies of cytokines and chemokines will improve our understanding of soluble mediators and potential targets for herpetic uveitis.
报告两名疱疹性葡萄膜炎患者眼内液的细胞因子/趋化因子谱。
对两名疱疹性葡萄膜炎患者进行眼内液细胞因子和趋化因子分析。将眼内液检查结果与疾病表现及患者临床病程相关联。
病例1为一名45岁女性，因右眼反复发红11天、视力下降前来就诊，被诊断为急性视网膜坏死。经前房穿刺获取的眼内液经聚合酶链反应（PCR）检测，水痘带状疱疹病毒（VZV）呈阳性。随后，该患者发展为增殖性玻璃体视网膜病变，需要进行玻璃体切割术。眼内液样本细胞因子/趋化因子分析检测到干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）、白细胞介素-18（IL-18）、巨噬细胞炎性蛋白-1β（MIP-1β）、干扰素诱导蛋白10（IP-10）和单核细胞趋化蛋白-1（MCP-1），其中MCP-1是含量最丰富的细胞因子。病例2为一名30岁女性，右眼渐进性疼痛伴视力下降两个月。经诊断性前房穿刺后，她被诊断为高血压性前葡萄膜炎。尽管前葡萄膜炎治疗成功，但她的眼压仍持续升高，需要进行青光眼滤过手术。在手术时采集眼内液样本进行细胞因子/趋化因子谱分析，检测到7种细胞因子/趋化因子水平，包括白细胞介素-6（IL-6）、IL-8、IL-18、MIP-1β、IP-10、MCP-1和白细胞介素-1受体拮抗剂（IL-1RA），其中IL-1RA是含量最丰富的细胞因子。
两名疱疹性葡萄膜炎患者的细胞因子/趋化因子谱显示MCP-1、IP-10、IL-8和IL-18水平升高，而IL-1RA在高血压性前葡萄膜炎的慢性期升高。对细胞因子和趋化因子的进一步研究将增进我们对疱疹性葡萄膜炎可溶性介质及潜在靶点的理解。
