非小细胞肺癌中NUP155与NDC1的相互作用：肿瘤进展的一个有前景的靶点。
NUP155 and NDC1 interaction in NSCLC: a promising target for tumor progression.
作者信息
Li Kai-Min, Meng Li-Fei, Yang Zhi-Hao, Hu Wen-Tao
机构信息
Department of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, Ningbo, China.
出版信息
Front Pharmacol. 2024 Dec 10;15:1514367. doi: 10.3389/fphar.2024.1514367. eCollection 2024.
BACKGROUND
NUP155 was reported to involve breast invasive carcinoma and hepatocellular carcinoma. We hypothesized that NUP155 and NDC1impacted the progression of NSCLC.
METHODS
The dataset was analyzed to find differentially expressed genes. Functional enrichment analysis and Kaplan-Meier survival analysis were performed for differentially expressed genes. Western blot, Clone formation assay, Transwell assay and CCK-8 assay were performed to determine the performance and role of the target gene in NSCLC.
RESULTS
The research found that the NUP family played a role in various diseases. Differential expression analysis and survival analysis were performed to identify 6 related-genes, including NUP155, NDC1, KPNA2, MAD2L1, NUP62CL, and POM121L2NUP155 and NDC1 could interact with NUP53, respectively. This effect was necessary to complete the assembly of the nuclear pore complex.
CONCLUSION
NUP155 interacted with NDC1 to complete the assembly of the nuclear pore complex, which promoted the development of NSCLC. Our study demonstrated that NUP155 was expected to be a potential target for the treatment of NSCLC.
背景
据报道，NUP155与乳腺浸润性癌和肝细胞癌有关。我们推测NUP155和NDC1影响非小细胞肺癌（NSCLC）的进展。
方法
分析数据集以寻找差异表达基因。对差异表达基因进行功能富集分析和Kaplan-Meier生存分析。进行蛋白质免疫印迹法、克隆形成试验、Transwell试验和CCK-8试验，以确定目标基因在NSCLC中的表现和作用。
结果
研究发现核孔蛋白（NUP）家族在多种疾病中起作用。进行差异表达分析和生存分析以鉴定6个相关基因，包括NUP155、NDC1、核转运蛋白α2（KPNA2）、有丝分裂后期促进复合物2（MAD2L1）、NUP62CL和POM121L2。NUP155和NDC1可分别与NUP53相互作用。这种作用对于完成核孔复合体的组装是必要的。
结论
NUP155与NDC1相互作用以完成核孔复合体的组装，从而促进NSCLC的发展。我们的研究表明，NUP155有望成为NSCLC治疗的潜在靶点。
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