Rodríguez-Martín Noelia M, Márquez-López José Carlos, González-Jurado José Antonio, Millán Francisco, Pedroche Justo, Fernández-Pachón María-Soledad
Instituto de la Grasa-CSIC, Plant Protein Group, Seville 41013, Spain.
Área de Educación Física y Deportiva, Departamento del Deporte e Informática, Universidad Pablo de Olavide, Carretera de Utrera Km 1, Seville 41013, Spain.
Biomed Pharmacother. 2025 Jan;182:117794. doi: 10.1016/j.biopha.2024.117794. Epub 2024 Dec 24.
The uncontrolled overproduction of Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) is linked to chronic inflammation, although they are also essential signaling molecules for the immune system against infectious agents. Bioactive compounds hold promise as functional bioactive nutrients, contributing to the immunomodulatory response. This study investigates the potential of chickpea protein hydrolysate to modulate ROS/RNS stress and inflammatory responses in a cellular low-grade chronic inflammatory model. This study was focused on their effects on endogenous antioxidant enzyme activities and key pro-inflammatory markers. ROS and nitric oxide (NO) production and molecular biology techniques were used to evaluate cell metabolism. Hydrolysate exposure notably increased ROS and NO release in a dose-dependent manner, while also exhibiting significant anti-inflammatory effects by inhibiting NF-κB and NLRP3 inflammasome components in treated cells. Therefore, chickpea protein hydrolysates hold promise as functional bioactive compounds for use in therapeutic applications, promoting human health and well-being.
活性氧（ROS）和活性氮（RNS）的失控过度产生与慢性炎症有关，尽管它们也是免疫系统对抗感染因子的重要信号分子。生物活性化合物有望作为功能性生物活性营养素，有助于免疫调节反应。本研究调查了鹰嘴豆蛋白水解物在细胞低度慢性炎症模型中调节ROS/RNS应激和炎症反应的潜力。本研究重点关注其对内源性抗氧化酶活性和关键促炎标志物的影响。采用ROS和一氧化氮（NO）产生及分子生物学技术来评估细胞代谢。水解物暴露显著以剂量依赖方式增加ROS和NO释放，同时通过抑制处理细胞中的NF-κB和NLRP3炎性小体成分表现出显著的抗炎作用。因此，鹰嘴豆蛋白水解物有望作为功能性生物活性化合物用于治疗应用，促进人类健康和福祉。
