Zhao Si-Yu, Chen Yan, Niu Xing-Yi, Zhang Yu, Liu Yong-Qi, Ding Chuan-Yu, Bai-Lian Larry Li, Duan Peng-Fei, Chen Zhao-Jin
School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China.
College of Civil Engineering and Architecture, Nanyang Normal University, Nanyang 473061, China.
Huan Jing Ke Xue. 2025 Jan 8;46(1):560-569. doi: 10.13227/j.hjkx.202401094.
The coexistence of microplastics and heavy metals in soil can lead to more intricate environmental effects. While plant growth-promoting bacteria have been widely recognized for enhancing the remediation of heavy metal-contaminated soils, little research has been conducted to investigate whether they can alleviate the stress of microplastic-heavy metal composite contamination on plants. We investigated the effects of isolated and screened plant growth-promoting bacteria on the growth and cadmium （Cd） accumulation of under the composite pollution of Cd and polypropylene （PP） with different particle sizes （6.5 and 830 μm） in pot experiments and analyzed their effects on the composition of rhizosphere bacterial communities using high-throughput sequencing. Seven strains of bacteria were isolated and screened from soil contaminated with heavy metal-microplastic composites, identified as and spp. All exhibited plant growth-promoting characteristics, including IAA production, siderophores, phosphorus solubilization, and potassium solubilization. Inoculation of plant growth-promoting bacteria increased the length and dry weight of , effectively alleviating the stress caused by PP+Cd compound pollution. Plant growth-promoting bacteria increased soil available potassium and available phosphorus content, mitigating the decrease in soil mineral nutrients caused by PP+Cd composite pollution. PP+Cd compound pollution and plant growth-promoting bacterial inoculation affected the composition of the rhizosphere bacterial community of , influencing dominant populations such as Proteobacteria, Firmicutes, and Actinobacteria. This study observed that the isolated and screened plant growth-promoting bacteria can effectively alleviate the plastic-heavy metal complex pollution caused by This provides a theoretical basis and data support for the remediation of microplastic heavy metal complex-contaminated soil biological pollution.
土壤中微塑料和重金属的共存会导致更复杂的环境影响。虽然促进植物生长的细菌在增强重金属污染土壤的修复方面已得到广泛认可，但关于它们是否能减轻微塑料-重金属复合污染对植物的胁迫的研究却很少。我们通过盆栽试验研究了分离筛选出的促进植物生长的细菌对在不同粒径（6.5和830μm）的镉（Cd）和聚丙烯（PP）复合污染下的植物生长和镉积累的影响，并使用高通量测序分析了它们对根际细菌群落组成的影响。从重金属-微塑料复合污染的土壤中分离筛选出7株细菌，鉴定为芽孢杆菌属和假单胞菌属。所有菌株均表现出促进植物生长的特性，包括产生吲哚乙酸、铁载体、解磷和解钾。接种促进植物生长的细菌增加了植物的长度和干重，有效缓解了PP+Cd复合污染造成的胁迫。促进植物生长的细菌增加了土壤有效钾和有效磷含量，减轻了PP+Cd复合污染导致的土壤矿质养分下降。PP+Cd复合污染和接种促进植物生长的细菌影响了植物根际细菌群落的组成，影响了变形菌门、厚壁菌门和放线菌门等优势种群。本研究观察到，分离筛选出的促进植物生长的细菌能有效缓解PP造成的塑料-重金属复合污染，这为微塑料重金属复合污染土壤生物修复提供了理论依据和数据支持。
