Fu Yansong, Wang Juexuan, Su Ziwei, Chen Qinyuan, Li Jiaxin, Zhao Junwei, Xuan Wei, Miao Youzhi, Zhang Ji, Zhang Ruifu
Key Lab of Organic-based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
College of Plant Protection, Northeast Agricultural University, Harbin, 150030, China.
New Phytol. 2025 Mar;245(5):2016-2037. doi: 10.1111/nph.20370. Epub 2024 Dec 26.
Sinomonas species typically reside in soils or the rhizosphere and can promote plant growth. Sinomonas enrichment in rhizospheric soils is positively correlated with increases in plant biomass. However, the growth promotion mechanisms regulated by Sinomonas remain unclear. By using soil systems, we studied the growth-promoting effects of Sinomonas gamaensis NEAU-HV1 on various plants. Through a combination of phenotypic analyses and microscopic observations, the effects of NEAU-HV1 on root development were evaluated. We subsequently conducted molecular and genetic experiments to reveal the mechanism promoting lateral root (LR) development. We demonstrated that NEAU-HV1 significantly promoted the growth of lettuce, wheat, maize, peanut and Arabidopsis. This effect was associated with multiple beneficial traits, including phosphate solubilization, indole-3-acetic acid and 1-aminocyclopropane-1-carboxylic acid deaminase production and survival ability in the rhizosphere and within the inner tissue of roots. In addition, NEAU-HV1 could secrete metabolites to promote LR development by affecting auxin transport and signaling. Importantly, we found that the influence of auxin signaling may be attributed to the remodeling interaction between SOLITARY-ROOT (SLR)/IAA14 and ARF7/19, occurring independently of the auxin receptor TIR1/AFB2. Our results indicate that NEAU-HV1-induced LR formation is dependent on direct remodeling interactions between transcription factors, providing novel insights into plant-microbe interactions.
中华单胞菌属通常存在于土壤或根际中，能够促进植物生长。根际土壤中中华单胞菌的富集与植物生物量的增加呈正相关。然而，中华单胞菌调控的植物生长促进机制仍不清楚。通过利用土壤系统，我们研究了中华单胞菌γ亚种NEAU-HV1对多种植物的促生长作用。通过表型分析和显微镜观察相结合的方法，评估了NEAU-HV1对根系发育的影响。随后，我们进行了分子和遗传实验，以揭示促进侧根（LR）发育的机制。我们证明NEAU-HV1显著促进了生菜、小麦、玉米、花生和拟南芥的生长。这种作用与多种有益特性相关，包括解磷、吲哚-3-乙酸和1-氨基环丙烷-1-羧酸脱氨酶的产生以及在根际和根内部组织中的存活能力。此外，NEAU-HV1可以分泌代谢产物，通过影响生长素运输和信号传导来促进侧根发育。重要的是，我们发现生长素信号的影响可能归因于单根（SLR）/IAA14和ARF7/19之间的重塑相互作用独立于生长素受体TIR1/AFB2发生。我们的结果表明，NEAU-HV1诱导的侧根形成依赖于转录因子之间的直接重塑相互作用，为植物-微生物相互作用提供了新的见解。
