Zhao Hongyu, Wang Yu, Sun Xueyuan, He Xingyao, Di Lin, Yang Zhen
Key Laboratory of TCM Pharmacology Jilin Academy of Chinese Medicine Sciences Changchun Jilin P.R. China.
College of Chemistry and Life Sciences Changchun University of Technology Changchun Jilin P.R. China.
Food Sci Nutr. 2024 Dec 4;12(12):10946-10961. doi: 10.1002/fsn3.4621. eCollection 2024 Dec.
The development status of follicles determines the menstrual cycle and estrogen levels, which is crucial to women's health. is a natural product for both medicine and food, which has "estrogenic effect". However, few studies have systematically elaborated its mechanism of action. Hence, we hypothesize the "estrogen-like effects" of OR may stem from its positive influence on the growth and development of growing follicles in the ovaries. In this study, the effect of (OR) on the growth and development of rat follicles and follicles cultured in vitro was investigated. The content of estrogen in rat serum and follicular culture medium in vitro was determined by radioimmunoassay, and the levels of PI3K-Akt signal pathway and FSHR expression in rat ovary and cultured follicles were detected by RT-PCR and Western Blot. The follicles at different developmental stages in rat ovaries were analyzed by H&E and TUNEL staining. Follicles cultured in a medium containing OR displayed a significant increase in diameter, and the E2 content in the medium was significantly increased. Moreover, Rats treated with OR showed significant increases in estradiol and progesterone levels. The number of antral follicles in the ovaries displayed a significant increase, while the percentage of atretic antral follicles and the total atretic follicles showed significant decreases. The relative expression levels of PI3K, Akt, and FSHR were significantly increased, while significantly decreased in that of PTEN In Vivo and In Vitro. OR may promote follicle growth through PI3K/ Akt pathway, exhibiting "estrogen-like effects".
卵泡的发育状态决定月经周期和雌激素水平，这对女性健康至关重要。（此处原文“is a natural product for both medicine and food”缺少主语，无法准确翻译，推测可能是某种物质，假设为“OR”）OR是一种药食同源的天然产物，具有“雌激素样作用”。然而，很少有研究系统阐述其作用机制。因此，我们推测OR的“雌激素样作用”可能源于其对卵巢中生长卵泡生长发育的积极影响。本研究探讨了OR对大鼠卵泡及体外培养卵泡生长发育的影响。采用放射免疫分析法测定大鼠血清和体外卵泡培养基中雌激素含量，通过RT-PCR和Western Blot检测大鼠卵巢和培养卵泡中PI3K-Akt信号通路及FSHR表达水平。采用H&E和TUNEL染色分析大鼠卵巢中不同发育阶段的卵泡。在含OR的培养基中培养的卵泡直径显著增加，培养基中E2含量显著升高。此外，用OR处理的大鼠雌二醇和孕酮水平显著升高。卵巢中窦状卵泡数量显著增加，而闭锁窦状卵泡百分比和闭锁卵泡总数显著减少。体内和体外PI3K、Akt和FSHR的相对表达水平显著升高，而PTEN的相对表达水平显著降低。OR可能通过PI3K/Akt途径促进卵泡生长，表现出“雌激素样作用”。
