Dai Wenshan, Pan Mengni, Peng Liqin, Zhang Di, Ma Yue, Wang Min, Wang Nian
National Navel Orange Engineering Research Center, College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
Jiangxi Provincial Key Laboratory of Pest and Disease Control of Featured Horticultural Plants, Gannan Normal University, Ganzhou 341000, China.
J Agric Food Chem. 2025 Jan 8;73(1):919-937. doi: 10.1021/acs.jafc.4c08160. Epub 2024 Dec 26.
Salicylic acid (SA) exhibits positive effects against Huanglongbing (HLB), but how SA affects citrus resistance to HLB is currently unknown. This study conducted integrated transcriptome and metabolome analyses on SA-treated (HLB-sensitive) and (HLB-tolerant). The results indicated that the syntheses of flavones and flavonols were induced by SA, while the expression levels of associated genes and the contents of corresponding metabolites varied significantly between the two species after SA treatment or HLB infection. These differences may underpin the enhanced HLB management through SA treatment and the inherent HLB tolerance of . Furthermore, two insertions of miniature inverted-repeat transposable element (MITE) were identified within the promoter of in , whereas none were found in the promoter of in . These MITE insertions notably enhanced the promoter activity of in an SA-dependent manner. Our findings deepen the understanding of the correlation between SA response and HLB tolerance in .
水杨酸（SA）对黄龙病（HLB）具有积极作用，但目前尚不清楚SA如何影响柑橘对HLB的抗性。本研究对经SA处理的（HLB敏感型）和（HLB耐受型）进行了转录组和代谢组综合分析。结果表明，黄酮类和黄酮醇类的合成由SA诱导，而SA处理或HLB感染后，两个品种中相关基因的表达水平和相应代谢物的含量存在显著差异。这些差异可能是通过SA处理增强HLB管理以及（品种名缺失）对HLB固有耐受性的基础。此外，在（品种名缺失）的（基因名缺失）启动子内鉴定出两个微型反向重复转座元件（MITE）插入，而在（品种名缺失）的（基因名缺失）启动子中未发现此类插入。这些MITE插入以SA依赖的方式显著增强了（基因名缺失）的启动子活性。我们的研究结果加深了对（品种名缺失）中SA反应与HLB耐受性之间相关性的理解。
