Wang Xin, Huang Jen-How, Meng Bo, Mao Kang, Zheng Mengmeng, Tan Aijuan, Yang Guili, Feng Xinbin
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, Guizhou, China.
Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, Guizhou, China.
Environ Sci Technol. 2025 Feb 11;59(5):2711-2721. doi: 10.1021/acs.est.4c08749. Epub 2024 Dec 26.
Glutathione S-transferase (GST) has been established to play an important role in regulating the responses of plants to stress, although its function and mechanisms of action in the cadmium (Cd)-tolerant remain unclear. In this study, we sought to identify a Cd-responsive gene from for functional analysis and mechanistic characterization. We accordingly identified a member of the gene family, , which plays a positive role in adaptation of to Cd. Having successfully obtained overexpressing (OE) strains via genetic transformation, we established that these strains were characterized by elevated Cd tolerance compared with the wild-type strain, as evidenced by significant increases in growth rate, chlorophyll content, antioxidant enzyme activities, and Cd removal rate. At the transcriptome level, the OE strains were found to have a stronger regulatory ability in response to Cd, particularly with respect to photoprotection, antioxidant defense, and glycolytic metabolism, which may be key factors contributing to the Cd tolerance of . Our findings provide a basis for further elucidating the biochemical and molecular mechanisms underlying the Cd tolerance conferred by genes in and will potentially contribute to the utilization of in remediating aquatic pollution.
谷胱甘肽S-转移酶（GST）已被证实对调节植物对胁迫的反应起着重要作用，尽管其在耐镉方面的功能和作用机制仍不清楚。在本研究中，我们试图从[具体植物名称]中鉴定一个镉响应基因，用于功能分析和作用机制表征。我们据此鉴定出了[基因名称]基因家族的一个成员，[具体基因名称]，它在[植物名称]适应镉胁迫方面发挥着积极作用。通过遗传转化成功获得过表达（OE）菌株后，我们发现这些菌株与野生型菌株相比，具有更高的耐镉性，这表现为生长速率、叶绿素含量、抗氧化酶活性和镉去除率显著提高。在转录组水平上，发现OE菌株对镉胁迫具有更强的调节能力，特别是在光保护、抗氧化防御和糖酵解代谢方面，这可能是[植物名称]耐镉的关键因素。我们的研究结果为进一步阐明[植物名称]中[基因名称]基因赋予耐镉性的生化和分子机制提供了基础，并可能有助于利用[植物名称]修复水体污染。
