Jaeger Morgane, Vincentelli Renaud, Lasserre Rémi
Aix Marseille Université, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, CIML, Turing Center for Living Systems, Marseille, France.
Aix Marseille Université, Centre National de la Recherche Scientifique, AFMB, Marseille, France.
Methods Mol Biol. 2025;2840:163-174. doi: 10.1007/978-1-0716-4047-0_12.
Optogenetics often requires genetic modification of the target cells to enable the expression of specific optogenetic tools, making it difficult to study primary cells in their native state. We have recently generated a fully extracellular optogenetic system for reversible light control of T cell receptor (TCR) activation on murine naïve T cells, a cell model that is very difficult to manipulate genetically. This molecular system is very versatile and can be easily modified to study different cell systems in different species. Here we describe how to produce and use this extracellular optogenetic system to manipulate primary T cell activation.
光遗传学通常需要对靶细胞进行基因改造，以使其能够表达特定的光遗传学工具，这使得研究处于天然状态的原代细胞变得困难。我们最近开发了一种完全细胞外的光遗传学系统，用于对小鼠幼稚T细胞上的T细胞受体（TCR）激活进行可逆的光控，而小鼠幼稚T细胞是一种很难进行基因操作的细胞模型。这个分子系统非常通用，可以很容易地进行修改，以研究不同物种的不同细胞系统。在这里，我们描述了如何生产和使用这种细胞外光遗传学系统来操纵原代T细胞的激活。
