Khosronejad Arya, Arabion Hamidreza, Iraji Aida, Mokhtarzadegan Mohamad, Daneshi Seyyed Sajad, Asadi-Yousefabad Seyedeh-Leili, Zare Shahrokh, Nowzari Fariborz, Abbaspour Shekofeh, Akbarizadeh Fatemeh, Aliabadi Ehsan, Amiri Mohammad Amin, Zarei Moein, Ebrahimi Reyhaneh, Mussin Nadiar M, Kurmanalina Madina A, Tanideh Nader, Tamadon Amin
Department of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Science, Shiraz, Iran.
Department of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Science, Shiraz, Iran.
Tissue Cell. 2025 Apr;93:102700. doi: 10.1016/j.tice.2024.102700. Epub 2024 Dec 24.
Addressing mandibular defects poses a significant challenge in maxillofacial surgery. Recent advancements have led to the development of various biomimetic composite scaffolds aimed at facilitating mandibular defect reconstruction. This study aimed to assess the regenerative potential of a novel composite scaffold consisting of polylactic acid (PLA), hydroxyapatite nanoparticles (n-HA), gelatin, hesperidin, and human dental pulp stem cells (DPSCs) in a rat model of mandibular bone defect. The PLA-HA-GLA composite was synthesized using solvent casting-leaching and freeze-drying methods and subsequently treated with 11 mg of hesperidin. The physicochemical properties of the PLA-HA-GLA and PLA-HA-GLA-HIS composites were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and thermal gravimetric analysis (TGA). Additionally, the mechanical properties and cytotoxicity of DPSCs were assessed. Subsequently, PLA-HA-GLA and PLA-HA-GLA-HIS scaffolds with or without DPSCs were implanted into mandibular bone defects in rats, followed by histopathological, histomorphometric, and cone-beam computed tomography (CBCT) evaluations after eight weeks. SEM analysis revealed the porous structure of the fabricated PLA-HA-GLA and PLA-HA-GLA-HIS composites without aggregation. FTIR and XRD analyses confirmed the presence of functional groups and elements associated with PLA, HA, GLA, and hesperidin in the composites. Although the PLA-HA-GLA-HIS composite exhibited good thermal stability, its mechanical properties decreased after the addition of hesperidin. The cell viability of DPSCs on the surface of the PLA-HA-GLA-HIS scaffolds was statistically significant compared to that of the control group. Furthermore, histopathological, histomorphometric, and radiological evaluations demonstrated that the implantation of the DPSC-loaded PLA-HA-GLA-HIS scaffold had a beneficial effect on bone tissue reconstruction in rats with mandibular defects. These findings highlight the potential of DPSC-loaded PLA-HA-GLA-HIS composite scaffolds for spongy bone tissue engineering and mandibular defect repair.
在颌面外科手术中，修复下颌骨缺损是一项重大挑战。近年来的进展促使人们开发出各种仿生复合支架，旨在促进下颌骨缺损的重建。本研究旨在评估一种新型复合支架的再生潜力，该支架由聚乳酸（PLA）、羟基磷灰石纳米颗粒（n-HA）、明胶、橙皮苷和人牙髓干细胞（DPSC）组成，用于大鼠下颌骨缺损模型。采用溶剂浇铸-浸出法和冷冻干燥法合成PLA-HA-GLA复合材料，随后用11 mg橙皮苷进行处理。通过扫描电子显微镜（SEM）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和热重分析（TGA）对PLA-HA-GLA和PLA-HA-GLA-HIS复合材料的物理化学性质进行分析。此外，还评估了DPSC的力学性能和细胞毒性。随后，将含或不含DPSC的PLA-HA-GLA和PLA-HA-GLA-HIS支架植入大鼠下颌骨缺损处，8周后进行组织病理学、组织形态计量学和锥形束计算机断层扫描（CBCT）评估。SEM分析显示，制备的PLA-HA-GLA和PLA-HA-GLA-HIS复合材料具有多孔结构且无聚集现象。FTIR和XRD分析证实了复合材料中存在与PLA、HA、GLA和橙皮苷相关的官能团和元素。虽然PLA-HA-GLA-HIS复合材料表现出良好的热稳定性，但添加橙皮苷后其力学性能有所下降。与对照组相比，DPSC在PLA-HA-GLA-HIS支架表面的细胞活力具有统计学意义。此外，组织病理学、组织形态计量学和放射学评估表明，植入载有DPSC的PLA-HA-GLA-HIS支架对下颌骨缺损大鼠的骨组织重建具有有益作用。这些发现突出了载有DPSC的PLA-HA-GLA-HIS复合支架在松质骨组织工程和下颌骨缺损修复方面的潜力。
