Alrshedan Abdulelah, Elsafadi Mona, Muthurangan Manikandan, Al-Hadlaq Solaiman
Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia.
Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.
Curr Issues Mol Biol. 2024 Dec 11;46(12):13979-13990. doi: 10.3390/cimb46120836.
Dental caries is a highly prevalent chronic disease that leads to dental pulp inflammation. It is treated by removing the damaged tooth structure and applying a material that promotes resolution of pulpal inflammation. Tumor necrosis factor superfamily 14 (TNFSF14) is an immunomodulatory cytokine and a member of the TNF superfamily. This study aimed to evaluate the effect of TNFSF14 on the levels of inflammatory cytokines involved in pulpal inflammation using lipoteichoic acid (LTA)-induced human dental pulp stem cells (hDPSCs). hDPSCs were cultured and induced with LTA, followed by treatment with TNFSF14 at 25 and 50 ng/mL. Cellular viability was evaluated using the Alamar Blue assay. The levels of inflammatory cytokines IL-6, IL-8, IL-10, and TNF-α were quantified using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). TNFSF14 at 25 and 50 ng/mL significantly reduced the mRNA and protein levels of pro-inflammatory cytokines , , and , and increased the anti-inflammatory cytokine . In addition, TNFSF14-treated groups enhanced cell viability. Adding TNFSF14 to LTA-induced hDPSCs regulated the production of inflammatory cytokines by lowering the levels of IL-6, IL-8, and TNF-α and elevating IL-10 levels.
龋齿是一种高度流行的慢性疾病，可导致牙髓炎症。其治疗方法是去除受损的牙齿结构，并应用一种能促进牙髓炎症消退的材料。肿瘤坏死因子超家族14（TNFSF14）是一种免疫调节细胞因子，属于TNF超家族成员。本研究旨在使用脂磷壁酸（LTA）诱导的人牙髓干细胞（hDPSC）评估TNFSF14对牙髓炎症中相关炎性细胞因子水平的影响。hDPSC经培养并用LTA诱导，随后分别用25 ng/mL和50 ng/mL的TNFSF14进行处理。使用alamar蓝检测法评估细胞活力。使用逆转录-定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）对炎性细胞因子白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）的水平进行定量分析。25 ng/mL和50 ng/mL的TNFSF14显著降低了促炎细胞因子IL-6、IL-8和TNF-α的mRNA和蛋白水平，并增加了抗炎细胞因子IL-10的水平。此外，TNFSF14处理组提高了细胞活力。向LTA诱导的hDPSC中添加TNFSF14可通过降低IL-6、IL-8和TNF-α水平以及提高IL-10水平来调节炎性细胞因子的产生。
