Garzón Ingrid, Muñoz-Hurtado Juan, Pereira-Martínez Juan, Ionescu Ana M, Cardona Juan de la Cruz, Tejada-Casado María, Pérez María Del Mar, Campos Fernando, Chato-Astrain Jesús, Alaminos Miguel
Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, E18016 Granada, Spain.
Instituto de Investigación Biosanitaria ibs.GRANADA, E18011 Granada, Spain.
Mar Drugs. 2024 Nov 28;22(12):535. doi: 10.3390/md22120535.
Cornea tissue engineering is strictly dependent on the development of biomaterials that fulfill the strict biocompatibility, biomechanical, and optical requirements of this organ. In this work, we generated novel biomaterials from the squid gladius (SG), and their application in cornea tissue engineering was evaluated. Results revealed that the native SG (N-SG) was biocompatible in laboratory animals, although a local inflammatory reaction was driven by the material. Cellularized biomaterials (C-SG) demonstrated that the SG provides an adequate substrate for cell attachment and growth, and corneal epithelial cells cultured on this biomaterial were able to express crystallin alpha, a marker for this type of cells. Biomechanical analyses showed that N-SG biomaterials have higher Young modulus and lower traction deformation than control native corneas (CTR), and C-SG showed a similar Young modulus than CTR. Analysis of the optical properties of these samples revealed that the diffuse transmittance of N-SG and C-SG were higher than CTR, with the diffuse reflectance showing the opposite behavior. These results confirm the putative usefulness of this abundant marine-derived biomaterial that can be obtained as a byproduct of the fishing industry.
角膜组织工程在很大程度上依赖于能够满足该器官严格的生物相容性、生物力学和光学要求的生物材料的发展。在这项工作中，我们从鱿鱼角质内壳（SG）制备了新型生物材料，并评估了它们在角膜组织工程中的应用。结果表明，天然SG（N-SG）在实验动物中具有生物相容性，尽管该材料引发了局部炎症反应。细胞化生物材料（C-SG）表明，SG为细胞附着和生长提供了合适的基质，在这种生物材料上培养的角膜上皮细胞能够表达α-晶状体蛋白，这是这类细胞的一个标志物。生物力学分析表明，N-SG生物材料比对照天然角膜（CTR）具有更高的杨氏模量和更低的拉伸变形，而C-SG显示出与CTR相似的杨氏模量。对这些样品光学性质的分析表明，N-SG和C-SG的漫透射率高于CTR，而漫反射率则表现出相反的趋势。这些结果证实了这种丰富的海洋来源生物材料作为渔业副产品可获得的潜在用途。
