Gong Yunxia, Li Shengfa, Zhao Deqing, Yuan Xi, Zhou Yin, Chen Fusheng, Shao Yanchun
College of Food Science and Technology, Wuhan Business University, Wuhan 430056, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
J Fungi (Basel). 2024 Dec 23;10(12):892. doi: 10.3390/jof10120892.
, a genus of fungi known for its fermentation capability and production of bioactive compounds, such as azaphilone pigments and Monacolin K, have received considerable attention because of their potential in biotechnological applications. Understanding the genetic basis of these metabolic pathways is crucial for optimizing the fermentation and enhancing the yield and quality of these products. However, spp. are not model fungi, and knowledge of their genetics is limited, which is a great challenge in understanding physiological and biochemical phenomena at the genetic level. Since the first application of particle bombardment to explore gene function, it has become feasible to link the phenotypic variation and genomic information on strains. In recent decades, accurate gene editing assisted by genomic information has provided a solution to analyze the functions of genes involved in the metabolism and development of spp. at the molecular level. This review summarizes most of the genetic manipulation tools used in spp. and emphasizes -mediated transformation and nuclease-guided gene editing, providing comprehensive references for scholars to select suitable genetic manipulation tools to investigate the functions of genes of interest in spp.
由于其在生物技术应用中的潜力，一个以发酵能力和生物活性化合物（如氮杂环庚三烯酮色素和莫纳可林K）生产而闻名的真菌属受到了广泛关注。了解这些代谢途径的遗传基础对于优化发酵以及提高这些产品的产量和质量至关重要。然而，该属真菌并非模式真菌，其遗传学知识有限，这在从基因层面理解生理和生化现象方面是一个巨大挑战。自从首次应用粒子轰击来探索基因功能以来，将该属菌株的表型变异与基因组信息联系起来已变得可行。近几十年来，由基因组信息辅助的精确基因编辑为在分子水平分析该属真菌代谢和发育相关基因的功能提供了解决方案。本综述总结了该属真菌中使用的大多数遗传操作工具，并重点介绍了介导转化和核酸酶引导的基因编辑，为学者选择合适的遗传操作工具来研究该属真菌中感兴趣基因的功能提供全面参考。
