Zhang Xudong, Wu Xiaochun, Zhang Yuting, Chen Yulu, Li Tingwen, Shi Yuan, Bao Shijun
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Vet Sci. 2024 Dec 10;11(12):639. doi: 10.3390/vetsci11120639.
(MS) is an important pathogen that can cause respiratory diseases in poultry and birds, leading to serious economic losses in the world and impacting the development of the poultry industry. However, the pathogenic mechanisms of MS infection are still unclear, resulting in a lack of effective diagnosis and treatment strategies. This study aimed to uncover the infection effect caused by MS in chicken oviduct cells. MS-infected chicken oviduct cells with different infection times and doses were collected, and DAPI staining was performed to monitor the morphological changes of cell nuclei. Cell viability was detect by CCK-8 assay. The expression of marker genes of cell apoptosis was determined by RT-qPCR and Western blotting assay, respectively. The results showed that MS infection significantly inhibited the growth of chicken oviduct cells, caused the nuclear shrinkage and rupture, activated the expression of apoptosis marker genes Caspase 3 and Beclin-1, and increased the apoptosis rate. These results indicated that MS infection inhibits cell proliferation and promotes apoptosis in chicken oviduct cells, which provided a basis for further revealing the MS pathogenic mechanism and provided a foundation for the future development of anti-infection strategies.
鸡毒支原体（MS）是一种重要的病原体，可导致家禽和鸟类的呼吸道疾病，在全球范围内造成严重的经济损失，并影响家禽业的发展。然而，MS感染的致病机制仍不清楚，导致缺乏有效的诊断和治疗策略。本研究旨在揭示MS对鸡输卵管细胞的感染效应。收集不同感染时间和剂量的MS感染鸡输卵管细胞，并进行DAPI染色以监测细胞核的形态变化。通过CCK-8法检测细胞活力。分别通过RT-qPCR和蛋白质免疫印迹法检测细胞凋亡标记基因的表达。结果表明，MS感染显著抑制鸡输卵管细胞的生长，导致细胞核萎缩和破裂，激活凋亡标记基因Caspase 3和Beclin-1的表达，并增加凋亡率。这些结果表明，MS感染抑制鸡输卵管细胞的增殖并促进其凋亡，为进一步揭示MS致病机制提供了依据，并为未来抗感染策略的开发奠定了基础。
