Kempski O, Spatz M, Valet G, Baethmann A
J Cell Physiol. 1985 Apr;123(1):51-4. doi: 10.1002/jcp.1041230109.
Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function.
作为具有高度生物学重要性的基本细胞功能，细胞体积调节在培养的脑血管内皮细胞中得到了研究。使用多参数流式细胞术系统能够同时测量细胞体积、活力以及膜电位或细胞内pH值。内皮细胞作为血脑屏障（BBB）的细胞成分，在暴露于低渗透压时会立即肿胀。这与膜去极化以及细胞内pH值下降有关。在30 - 60分钟内，尽管细胞外渗透压保持在低水平，但细胞体积和膜电位会完全恢复。细胞内pH值并未完全恢复正常。细胞内钾离子和钠离子浓度的测量结果显示它们参与了调节过程。这些发现有力地表明，脑血管内皮细胞具有对正常血脑屏障功能至关重要的高效内在稳态控制能力。
