利用环境DNA宏条形码技术检测和鉴定水样中的疫霉属真菌。
The Use of eDNA Metabarcoding to Detect and Identify Phytophthora in Water Samples.
作者信息
Randall Eva, Keillor Beatrix, Cooke David E L
机构信息
The James Hutton Institute, Dundee, UK.
出版信息
Methods Mol Biol. 2025;2892:117-138. doi: 10.1007/978-1-0716-4330-3_9.
PMID:39729273
Abstract
We describe a protocol to amplify DNA barcodes of known and unknown taxa of Phytophthora and related plant pathogenic oomycetes from a range of environments. The methods focus on sampling pathogen propagules from water using in situ sampling and filtration equipment and buffers that enable efficient storage and DNA extraction for later downstream processing.
摘要
我们描述了一种从一系列环境中扩增疫霉属及相关植物致病卵菌已知和未知分类群的DNA条形码的方案。这些方法侧重于使用原位采样和过滤设备以及能够实现高效储存和DNA提取以便后续下游处理的缓冲液，从水中对病原体繁殖体进行采样。
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