Dousti Mozhdeh, Mousavi Jafaripour Elham, Ahmadzadeh Maryam, Falahi Charkhabi Nargues, Ahmadzadeh Masoud
Department of Plant Protection, College of Agriculture, University College of Agriculture & Natural Resources, University of Tehran, Tehran, Iran.
Department of Research and Development, Razh Fadak Mehrshahr Company, Karaj, Iran.
Sci Rep. 2024 Dec 28;14(1):31011. doi: 10.1038/s41598-024-82167-3.
Agaricus bisporus is globally a most extensively consumed species of edible mushrooms. Ethylene secreted by A. bisporus mycelium suppress the initiation of fructification. 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing bacteria decrease the content of ethylene by cleaving ACC produced by the A. bisporus hyphae to α-ketobutyrate and ammonia. This study evaluated the effect of various salts on the population of Bacillus velezensis UTB96 with ACC deaminase activity on button mushroom. The results indicated that CaCl, MnSO and MgSO salts increased significantly the bacterial population compared by control, among which the medium supplemented with CaCl showed the highest number of living cells in the culture. Evaluation of ACC deaminase gene expression indicated that the highest level belonged to bacteria grown in medium containing CaCl in comparison with control as the expression level of ACC deaminase was 20.9-fold upregulated comparing to the control. The assessment of the effect of each salt lonely, UTB96 cultured in LB and LB containing salts of mushroom indicated that yield was increased 23% and 18% in plots treated with UTB96 cultured in media supplemented, respectively, with CaCl and MnSO, in comparison with control. Therefore, UTB96 grown in media supplemented with CaCl has a potential use in mushroom production since the induction of ACC deaminase which results in decrease the level of ethylene and promote mushroom growth.
双孢蘑菇是全球消费最广泛的食用蘑菇品种。双孢蘑菇菌丝体分泌的乙烯会抑制子实体的形成。产1-氨基环丙烷-1-羧酸（ACC）脱氨酶的细菌通过将双孢蘑菇菌丝体产生的ACC裂解为α-酮丁酸和氨来降低乙烯含量。本研究评估了各种盐对具有ACC脱氨酶活性的贝莱斯芽孢杆菌UTB96在双孢蘑菇上种群数量的影响。结果表明，与对照相比，CaCl、MnSO和MgSO盐显著增加了细菌种群数量，其中添加CaCl的培养基中培养的活细胞数量最多。对ACC脱氨酶基因表达的评估表明，与对照相比，在含有CaCl的培养基中生长的细菌中ACC脱氨酶基因表达水平最高，其ACC脱氨酶表达水平比对照上调了20.9倍。单独评估每种盐的效果，在LB和含有蘑菇盐的LB中培养UTB96，结果表明，与对照相比，用分别添加CaCl和MnSO的培养基培养的UTB96处理的地块产量分别提高了23%和18%。因此，在添加CaCl的培养基中生长的UTB96在蘑菇生产中具有潜在用途，因为诱导ACC脱氨酶可降低乙烯水平并促进蘑菇生长。
