Tian Danxing, Liu Wei, Cai Lijuan
Department of Internal Medicine of TCM, Tongde Hospital of Zhejiang Province, Hangzhou, 310012, Zhejiang, China.
Department of General Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, 310012, Zhejiang, China.
Mol Biotechnol. 2024 Dec 27. doi: 10.1007/s12033-024-01345-w.
This study aimed to investigate the protective effects of Lycium barbarum polysaccharide (LBP) on digestive function and intestinal barrier integrity in septic mice, and to explore its underlying mechanisms. A total of 70 healthy male BALB/C mice were randomly assigned into five groups: blank control group (BG, n = 12), control group (CG, n = 12), low-dose group (LDG, n = 12, 200 mg/kg), medium-dose group (MDG, n = 12, 400 mg/kg), and high-dose group (HDG, n = 12, 800 mg/kg). A sepsis model was established by cecal ligation and puncture, followed by treatment with different doses of LBP. Digestive function was assessed by measuring body weight, food intake, and stool consistency. Histopathological changes in the small intestine were observed via hematoxylin and eosin (H&E) staining, and the activation of the JNK signaling pathway was analyzed by Western blotting. One week after model establishment, a dose-dependent increase in body weight was observed in the LBP-treated mice (P < 0.05). The LBP treatment groups exhibited higher food intake and significantly lower stool wet weight compared to the CG (P < 0.05). Gastric emptying rate followed the order: BG > HDG > MDG > LDG > CG (P < 0.05). The intestinal propulsion rate was ranked from high to low as CG > LDG > MDG > HDG > BG, while intestinal absorption capacity was ranked from high to low as BG > HDG > MDG > LDG > CG (P < 0.05). The expression of RNA and protein associated with the JNK pathway showed the order: BG > HDG > MDG > LDG > CG, whereas the expression of MUC2 followed the opposite order (P < 0.05). LBP treatment significantly prolonged the survival time of the septic mice (P < 0.05). LBP significantly improves digestive function and intestinal barrier integrity in septic mice by modulating the inflammatory response and inhibiting JNK pathway activation.
本研究旨在探讨枸杞多糖（LBP）对脓毒症小鼠消化功能和肠道屏障完整性的保护作用，并探究其潜在机制。将70只健康雄性BALB/C小鼠随机分为五组：空白对照组（BG，n = 12）、对照组（CG，n = 12）、低剂量组（LDG，n = 12，200 mg/kg）、中剂量组（MDG，n = 12，400 mg/kg）和高剂量组（HDG，n = 12，800 mg/kg）。通过盲肠结扎和穿刺建立脓毒症模型，随后用不同剂量的LBP进行治疗。通过测量体重、食物摄入量和粪便稠度评估消化功能。通过苏木精-伊红（H&E）染色观察小肠的组织病理学变化，并通过蛋白质印迹法分析JNK信号通路的激活情况。模型建立一周后，在LBP治疗的小鼠中观察到体重呈剂量依赖性增加（P < 0.05）。与CG组相比，LBP治疗组的食物摄入量更高，粪便湿重显著更低（P < 0.05）。胃排空率顺序为：BG > HDG > MDG > LDG > CG（P < 0.05）。肠推进率从高到低依次为：CG > LDG > MDG > HDG > BG，而肠吸收能力从高到低依次为：BG > HDG > MDG > LDG > CG（P < 0.05）。与JNK通路相关的RNA和蛋白质表达顺序为：BG > HDG > MDG > LDG > CG，而MUC2的表达顺序则相反（P < 0.05）。LBP治疗显著延长了脓毒症小鼠的存活时间（P < 0.05）。LBP通过调节炎症反应和抑制JNK通路激活，显著改善了脓毒症小鼠的消化功能和肠道屏障完整性。
