Fan Chunli, Zhang Min, Hou Yaqi, Wang Xinshuo, Xia Shuqin, Fang Zhongxiang
State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China; School of Agriculture, Food and Ecosystem Sciences, Faculty of Science, The University of Melbourne, Parkville, Vic 3010, Australia.
State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Int J Biol Macromol. 2025 Mar;292:139226. doi: 10.1016/j.ijbiomac.2024.139226. Epub 2024 Dec 26.
This work examined the effects of molecular weight (2-15 kDa) and concentration (10-30 mg/mL) of chitosan (CTS) on the binding capacity and interface behavior between octenyl succinic acid sodium starch (OSS) and CTS, as well as their effects on the storage stability of emulsions. The results of the isothermal calorimetry titration demonstrated that OSS and CTS were complexed by electrostatic interaction and spontaneous hydrogen bonding driven by enthalpy (ΔH from -3931 to -7983 cal/mol, ΔS from -38.5 to -49.2 cal/mol/deg., ΔG from -6436.6 to -7542.0 cal/mol), and the affinity and binding capacity (N = 2.83-22.90 sites) were reinforced with CTS molecular weight. The interfacial adsorption kinetics revealed that the OSS/CTS complexes with high-molecular-weight CTS exhibited a markedly accelerated rate of interfacial diffusion and recombination, and rapidly reduced interfacial tension. CTS incorporation enhanced mass (1980.32-3522.56 ng/cm), thickness (35.12-51.29 nm), viscosity (52.81-67.06 kPa), and elasticity (1.70-3.49 kPa) of interfacial film through the dissipative quartz crystal microbalance. OSS/CTS complexes displayed superior emulsification properties and adjustable interfacial viscoelastic ratio in response to frequency interference. Emulsions stabilized by the OSS/CTS complexes with high molecular weight and concentration of CTS exhibited more uniform spherical structures and superior storage stability. This work will facilitate the broader utilization of OSS and CTS in the emulsion delivery system.
本研究考察了壳聚糖（CTS）的分子量（2 - 15 kDa）和浓度（10 - 30 mg/mL）对辛烯基琥珀酸淀粉钠（OSS）与CTS之间结合能力和界面行为的影响，以及它们对乳液储存稳定性的影响。等温滴定量热法滴定结果表明，OSS和CTS通过静电相互作用和焓驱动的自发氢键作用形成复合物（ΔH为 - 3931至 - 7983 cal/mol，ΔS为 - 38.5至 - 49.2 cal/mol/deg.，ΔG为 - 6436.6至 - 7542.0 cal/mol），且亲和力和结合能力（N = 2.83 - 22.90个位点）随CTS分子量的增加而增强。界面吸附动力学表明，具有高分子量CTS的OSS/CTS复合物表现出显著加速的界面扩散和重组速率，并迅速降低界面张力。通过耗散型石英晶体微天平研究发现，CTS的加入提高了界面膜的质量（1980.32 - 3522.56 ng/cm）、厚度（35.12 - 51.29 nm）、粘度（52.81 - 67.06 kPa）和弹性（1.70 - 3.49 kPa）。OSS/CTS复合物表现出优异的乳化性能，并能响应频率干扰调节界面粘弹性比。由具有高分子量和高浓度CTS的OSS/CTS复合物稳定的乳液呈现出更均匀的球形结构和优异的储存稳定性。本研究将有助于OSS和CTS在乳液递送系统中更广泛的应用。
