Ekinci Akdemir Fazile Nur, Güler Mustafa Can, Eraslan Ersen, Tanyeli Ayhan, Yildirim Serkan
Department of Nutrition and Dietetics, Faculty of Health Science, Ağrı İbrahim Çeçen University, Ağrı, Turkey.
Department of Physiology, Faculty of Medicine, Atatürk University, Erzurum, 25240, Turkey.
Sci Rep. 2024 Dec 28;14(1):31385. doi: 10.1038/s41598-024-82912-8.
Oxidative stress and inflammation are indispensable components of ischemia-reperfusion (IR) injury. In this study, we investigated the effects of low and high doses of caftaric acid (CA) on reducing kidney and remote organ damage induced by IR. We divided Wistar rats into four groups: sham, IR, low (40 mg/kg body weight (BW)), and high (80 mg/kg BW) CA groups. IR (1 h ischemia, 24 h reperfusion) was applied to all groups, except the sham one. Following the experimental period, we removed kidney and lung tissues to assess biochemical, histopathological, and immunohistochemical parameters. In the IR group, oxidant parameters (malondialdehyde (MDA), myeloperoxidase (MPO), total oxidant status (TOS), oxidative stress index (OSI)) increased, and antioxidant level parameters (superoxide dismutase (SOD) and total antioxidant status (TAS)) diminished. In addition, Microtubule-associated protein light chain 3 (LC3), cyclooxygenase-2 (COX-2), and caspase-3 immunopositivity were severe in the IR group. CA treatment improved the LC3, COX-2, and caspase-3 immunopositivity, lowered the oxidant level, and enhanced the antioxidant capacity. Histopathological findings were consistent with the data. In light of all our results, CA is effective against oxidative stress, autophagy, apoptosis, and inflammation in the renal IR experimental model.
氧化应激和炎症是缺血再灌注（IR）损伤必不可少的组成部分。在本研究中，我们研究了低剂量和高剂量的咖啡酸（CA）对减轻IR诱导的肾脏和远隔器官损伤的影响。我们将Wistar大鼠分为四组：假手术组、IR组、低剂量（40mg/kg体重（BW））CA组和高剂量（80mg/kg BW）CA组。除假手术组外，对所有组施加IR（1小时缺血，24小时再灌注）。实验期结束后，我们取出肾脏和肺组织以评估生化、组织病理学和免疫组化参数。在IR组中，氧化参数（丙二醛（MDA）、髓过氧化物酶（MPO）、总氧化状态（TOS）、氧化应激指数（OSI））升高，抗氧化水平参数（超氧化物歧化酶（SOD）和总抗氧化状态（TAS））降低。此外，微管相关蛋白轻链3（LC3）、环氧化酶-2（COX-2）和半胱天冬酶-3免疫阳性在IR组中严重。CA治疗改善了LC3、COX-2和半胱天冬酶-3免疫阳性，降低了氧化水平，并增强了抗氧化能力。组织病理学结果与数据一致。根据我们所有的结果，CA在肾脏IR实验模型中对氧化应激、自噬、凋亡和炎症有效。
