Basyegit Hilal, Tatar Bengu Gireniz, Kose Sukran, Gunduz Cumhur, Ozmen Yelken Besra, Yilmaz Susluer Sunde
Ege University Faculty of Medicine Department of Medical Biology, Izmir Turkey.
Izmir Tepecik Education and Research Hospital Clinic of Infectious Diseases, Izmir Turkey.
Asian Pac J Cancer Prev. 2024 Dec 1;25(12):4313-4321. doi: 10.31557/APJCP.2024.25.12.4313.
Hepatitis B is a significant public health issue worldwide. Long noncoding RNAs (lncRNAs) are pivotal in biological mechanisms. The involvement of lncRNAs in hepatitis Hepatitis B remains incompletely understood. This study sought to explore the potential of certain lncRNAs as diagnostic and prognostic markers in hepatitis B, and their ability to differentiate between clinical disease subgroups.
Plasma samples from 204 individuals diagnosed with hepatitis B were meticulously examined for the presence of specific lncRNAs, focusing on those known to be associated with hepatitis B as indicated in the literature. In this study, gene expression levels of 14 lncRNAs were analyzed in three subgroups (chronic, recovered and inactive HBsAg carriers) and compared with those in the healthy control group.
The downregulation of the RN7SL1 and Alpha-280 genes in all patients indicates potential diagnostic lncRNAs. ZFHX2-AS1 expression was consistently lower in all subgroups, while SRA1 showed high expression in recovered hepatitis B patients, indicating that it is a descriptive marker. DLG2-AS2 expression increased in the chronic hepatitis B but decreased in the other groups.
These findings reveal the prognostic importance of lncRNAs in hepatitis B. The expression levels of ZFHX2-AS1, RN7SL1, PCAT-1, PCA3, SRA1 and Alpha-280 have been identified as crucial biomarkers for the diagnosis of hepatitis B and for distinguishing between its clinical subgroups. A strong association between hepatitis B disease and the gene expression of the DLG2-AS2, HOTTIP, HOTAIRM1, HOXA11-AS, NOS2P3, LINC02665, MEG9, and RNY5 lncRNAs has been suggested.
乙型肝炎是全球重大的公共卫生问题。长链非编码RNA（lncRNA）在生物学机制中起关键作用。lncRNA在乙型肝炎中的作用仍未完全了解。本研究旨在探索某些lncRNA作为乙型肝炎诊断和预后标志物的潜力，以及它们区分临床疾病亚组的能力。
对204例诊断为乙型肝炎的个体的血浆样本进行了细致检查，以检测特定lncRNA的存在，重点关注文献中指出的与乙型肝炎相关的lncRNA。在本研究中，分析了14种lncRNA在三个亚组（慢性、康复和非活动性HBsAg携带者）中的基因表达水平，并与健康对照组进行比较。
所有患者中RN7SL1和Alpha-280基因的下调表明存在潜在的诊断性lncRNA。ZFHX2-AS1在所有亚组中的表达始终较低，而SRA1在康复的乙型肝炎患者中高表达，表明它是一个描述性标志物。DLG2-AS2在慢性乙型肝炎中表达增加，而在其他组中降低。
这些发现揭示了lncRNA在乙型肝炎中的预后重要性。已确定ZFHX2-AS1、RN7SL1、PCAT-1、PCA3、SRA1和Alpha-280的表达水平是诊断乙型肝炎及其临床亚组鉴别的关键生物标志物。已表明乙型肝炎疾病与DLG2-AS2、HOTTIP、HOTAIRM1、HOXA11-AS、NOS2P3、LINC02665、MEG9和RNY5 lncRNA的基因表达之间存在密切关联。
