Li Liang, Lv Muze, Li Yangfan, Sun Huihui, Li Jie, Li Wenyan, Wang Xuan, Bi Ruimin, Zhang Zuyao, Bo Zongyi, Shen Haixiao, Wang Jun, Zhuansun Minghao, Zhou Jinchi, Xue Yuting, Suo Xinru, Tong Rui, Sun Pei
College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Joint Research Center for Food Nutrition and Health of IHM, Anhui Agricultural University, Hefei, China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
Vet Microbiol. 2025 Feb;301:110356. doi: 10.1016/j.vetmic.2024.110356. Epub 2024 Dec 24.
Pseudorabies virus (PRV) is a significant pathogen that causes acute infectious diseases in pigs, resulting in considerable economic losses for the global pig industry. The lack of effective control measures and vaccines against the circulating variants of PRV highlights the pressing need for novel treatment strategies. In this study, a screening of a natural product library identified Berbamine as a promising compound that inhibits PRV replication, with a selectivity index of 17. Preliminary investigations demonstrated that Berbamine impedes viral proliferation by targeting the replication and release stages of the PRV life cycle. In experiments with mice artificially infected with PRV, Berbamine significantly alleviated clinical symptoms and histopathological changes in brain tissue caused by PRV infection. Furthermore, molecular docking studies indicated that Berbamine targets the UL50 protein, not only of PRV but also of HSV-1, FHV-1, and BoHV-1. Given that the UL50 protein is a promising target for antiviral drug development, Berbamine holds considerable potential for broad application in antiviral therapies.
伪狂犬病病毒（PRV）是一种重要的病原体，可导致猪的急性传染病，给全球养猪业造成巨大经济损失。缺乏针对PRV流行变异株的有效控制措施和疫苗凸显了对新型治疗策略的迫切需求。在本研究中，对天然产物文库进行筛选后，发现小檗胺是一种有前景的化合物，可抑制PRV复制，其选择性指数为17。初步研究表明，小檗胺通过靶向PRV生命周期的复制和释放阶段来阻碍病毒增殖。在人工感染PRV的小鼠实验中，小檗胺显著减轻了PRV感染引起的临床症状和脑组织的组织病理学变化。此外，分子对接研究表明小檗胺靶向UL50蛋白，不仅针对PRV，还针对单纯疱疹病毒1型（HSV-1）、禽副粘病毒1型（FHV-1）和牛疱疹病毒1型（BoHV-1）。鉴于UL50蛋白是抗病毒药物开发的一个有前景的靶点，小檗胺在抗病毒治疗中具有广泛应用的巨大潜力。
