Lietzke R, Unsicker K
J Immunol Methods. 1985 Feb 11;76(2):223-8. doi: 10.1016/0022-1759(85)90298-4.
One of the standard methods to isolate a hybridoma clone producing a monoclonal antibody requires successive steps of limiting dilution. The probability of obtaining a monoclonal antibody increases with the number of limiting dilution steps. However, without meticulous visual screening monoclonality is hard to prove. Here we describe a statistical analysis, based on Poisson's approximation, which allows one to calculate the number of hybridoma cells at a given plating efficiency so that when seeded a predictable number of mono-, bi-, etc.-clonal cultures are obtained after the first step of limiting cell plating.
分离产生单克隆抗体的杂交瘤克隆的标准方法之一需要连续进行有限稀释步骤。获得单克隆抗体的概率随着有限稀释步骤的数量增加而增加。然而，如果没有细致的目视筛选，单克隆性很难得到证明。在此，我们描述一种基于泊松近似的统计分析方法，该方法能够在给定接种效率的情况下计算杂交瘤细胞的数量，以便在进行有限细胞接种的第一步后，能够接种出可预测数量的单克隆、双克隆等克隆培养物。
