Rahman Md Kaisar, Rodriguez-Mori Howard, Loneragan Guy, Awosile Babafela
Texas Tech University School of Veterinary Medicine, Amarillo, TX, USA.
Texas Tech University School of Veterinary Medicine, Amarillo, TX, USA.
Int J Antimicrob Agents. 2025 Feb;65(2):107422. doi: 10.1016/j.ijantimicag.2024.107422. Epub 2024 Dec 27.
The study aimed to review the beta-lactamase resistance genes detected in Enterobacterales from humans, animals, and the environment in the United States.
We conducted a comprehensive search on PubMed, Web of Science, and Google Scholar for articles reporting beta-lactamase genes in the United States from 1981 to 22 April 2022, following the PRISMA protocol. Studies were evaluated based on predefined eligibility criteria, and both qualitative and quantitative analyses were conducted on the selected studies.
Of the 335 articles, a total of 169 different beta-lactamase genes, including narrow-spectrum, extended-spectrum, AmpC, and carbapenemase have been detected and reported in the United States, with human (137), animal (53), and environment (47). 22 genes (bla, bla, bla, bla, bla, bla, bla, bla, bla, bla) have been reported across animals, humans, and environment. Notably, bla was prevalent in E. coli isolates, with an overall pooled proportion of 10.7 %, varying between animals (8.6 %), humans (13.1 %), and the environment (0.8 %). Similarly, bla in E. coli isolates had an overall pooled proportion of 10.6 %, with distinctions in proportion among animals (1.6 %), humans (41.3 %), and the environment (16.2 %). The sequence type (ST131) was detected as the predominant, mainly associated with the bla, with a pooled proportion of 56.9 %, varying from 14.3 % to 90 % across studies.
This study highlights the distribution of beta-lactamases in the United States, essential for understanding One Health and the molecular epidemiology of key beta-lactamases, especially extended-spectrum beta-lactamases and carbapenemases.
本研究旨在回顾在美国从人类、动物和环境中分离出的肠杆菌科细菌中检测到的β-内酰胺酶耐药基因。
我们按照PRISMA方案，在PubMed、科学网和谷歌学术上进行了全面检索，以查找1981年至2022年4月22日期间在美国报道β-内酰胺酶基因的文章。根据预先设定的纳入标准对研究进行评估，并对所选研究进行定性和定量分析。
在335篇文章中，美国共检测并报道了169种不同的β-内酰胺酶基因，包括窄谱、广谱、AmpC和碳青霉烯酶基因，其中来自人类的有137种，动物的有53种，环境的有47种。有22种基因（bla、bla、bla、bla、bla、bla、bla、bla、bla、bla）在动物、人类和环境中均有报道。值得注意的是，bla在大肠杆菌分离株中普遍存在，总体合并比例为10.7%，在动物（8.6%）、人类（13.1%）和环境（0.8%）之间存在差异。同样，大肠杆菌分离株中的bla总体合并比例为10.6%，在动物（1.6%）、人类（41.3%）和环境（16.2%）中的比例也有所不同。序列类型（ST131）被检测为主要类型，主要与bla相关，合并比例为56.9%，在不同研究中从14.3%到90%不等。
本研究突出了美国β-内酰胺酶的分布情况，这对于理解“同一健康”以及关键β-内酰胺酶尤其是广谱β-内酰胺酶和碳青霉烯酶的分子流行病学至关重要。
