Jutte N H, Jansen R, Grootegoed J A, Rommerts F F, van der Molen H J
J Exp Zool. 1985 Feb;233(2):285-90. doi: 10.1002/jez.1402330217.
Isolated rat pachytene spermatocytes were incubated in chemically defined medium supplemented with pyruvate and lactate, which are known to be essential energy substrates for these germ cells. Protein synthesis by the isolated cells was investigated by means of two-dimensional polyacrylamide gel electrophoresis. The electrophoretic patterns of (35S)-labeled proteins, synthesized by the pachytene spermatocytes during incubation in the presence of (35S)methionine either from 0-2 h or from 24-26 h after isolation, were almost completely identical. The patterns of newly synthesized proteins of freshly isolated spermatocytes and spermatids, however, showed several stage-specific proteins in addition to many proteins common to both spermatogenic cell types. Hence, it was concluded that a stage-specific pattern of protein synthesis can be maintained by pachytene spermatocytes during incubation for a period of 24 h in the absence of Sertoli cells but in the presence of a proper energy source.
将分离出的大鼠粗线期精母细胞置于添加了丙酮酸和乳酸的化学成分明确的培养基中进行培养，已知丙酮酸和乳酸是这些生殖细胞必不可少的能量底物。通过二维聚丙烯酰胺凝胶电泳研究分离细胞的蛋白质合成情况。在分离后0 - 2小时或24 - 26小时期间，在存在(35S)甲硫氨酸的情况下，粗线期精母细胞合成的(35S)标记蛋白质的电泳图谱几乎完全相同。然而，新鲜分离的精母细胞和精子细胞新合成蛋白质的图谱显示，除了两种生精细胞类型共有的许多蛋白质外，还有几种阶段特异性蛋白质。因此，得出的结论是，在没有支持细胞但存在合适能量来源的情况下，粗线期精母细胞在培养24小时期间可以维持阶段特异性的蛋白质合成模式。
