Innocenti G M, Frost D O, Illes J
J Neurosci. 1985 Feb;5(2):255-67. doi: 10.1523/JNEUROSCI.05-02-00255.1985.
The number of callosally projecting neurons (callosal neurons) which can be labeled in cortical areas 17 and 18 by horseradish peroxidase (HRP), injected in the contralateral visual cortex, is reduced to about 50% of normal in cats reared with their eyelids bilaterally sutured. In the same animals the density of HRP anterogradely transported to areas 17 and 18 is also decreased. The apparent loss of callosal neurons is limited to layers III and IV (subzone a), whereas layer VI (subzone c) is unaffected. The effect is obtained after 3 months or more but not after 1 month of deprivation. Two months of visual experience following deprivation do not restitute a normal number of callosal neurons. However, 10 days of normal visual experience preceding the deprivation are sufficient to prevent the effects of the latter. Animals deprived of vision after a short period of normal visual experience and animals allowed normal vision after 1 month of visual deprivation have a more widespread distribution of callosal neurons than do normal animals; in this way they are similar to previously described cats reared with convergent or divergent strabismus, monocular enucleation, or monocular eyelid suture. The results suggest that: vision is actively responsible for both the maintenance and the elimination of fractions of the juvenile callosal connections; the elimination which normally takes place during the second postnatal month requires normal binocular vision; and activity-dependent competition between callosal and other axons can explain the role of vision.
将辣根过氧化物酶（HRP）注入对侧视皮层后，在17区和18区皮层中可被标记的胼胝体投射神经元（胼胝体神经元）数量，在双侧眼睑缝合饲养的猫中减少至正常数量的约50%。在相同的动物中，顺行运输至17区和18区的HRP密度也降低。胼胝体神经元的明显缺失仅限于III层和IV层（a亚区），而VI层（c亚区）未受影响。这种效应在剥夺3个月或更长时间后出现，但在剥夺1个月后未出现。剥夺后两个月的视觉经验并不能恢复正常数量的胼胝体神经元。然而，在剥夺前10天的正常视觉经验足以防止后者的影响。在短时间正常视觉经验后剥夺视觉的动物，以及在视觉剥夺1个月后给予正常视觉的动物，其胼胝体神经元的分布比正常动物更广泛；通过这种方式，它们类似于先前描述的患有会聚性或发散性斜视、单眼摘除或单眼眼睑缝合饲养的猫。结果表明：视觉对于维持和消除部分幼年胼胝体连接起着积极作用；通常在出生后第二个月发生的消除过程需要正常的双眼视觉；胼胝体轴突与其他轴突之间的活动依赖性竞争可以解释视觉的作用。
