Girmay Gebrerufael, Emeru Bezina Arega, Tegegne Desiye Tesfaye, Bora Shelema Kelbesa, Gudeta Workitu Firomsa, Dersso Betelhem Seyoum, Hurrisa Beksisa Urge, Werid Gebremeskel Mamu, Yalew Shimels Tikuye, Messele Yohannes Equar
Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P.O. Box: 249, Holeta, Ethiopia.
Ethiopian Institute of Agricultural Research, Holeta Agricultural Research Center, P.O. Box: 31, Holeta, Ethiopia.
BMC Res Notes. 2024 Dec 30;17(1):394. doi: 10.1186/s13104-024-07059-1.
The reproductive problem is an animal health-related bottleneck that constrains livestock genetic improvement efforts in tropical countries such as Ethiopia. The infectious causes of reproductive disorders are one cause of decreased reproductive efficiency. This study aimed to determine the seroprevalence to Bovine Herpesvirus-1 (BHV1), Bovine Viral Diarrhea Virus (BVDV), Neospora caninum (N. caninum) and C. burnetii (C. burnetii) exposures in dairy cows with reproductive disorders in selected areas of Ethiopia. Overall, 164 serum samples were collected from October 2018 to May 2019 from animals with a history of reproductive disorders. The collected sera were tested for antibody titers to Brucella species, N. caninum, BVDV, BHV1, C. burnetii and Chlamydophila abortus (C. abortus) using Rose Bengal and ELISA.
The apparent seroprevalence of BHV1, BVDV, N. caninum and C. burnetii were 61%, 33.5%, 4.9% and 0.6%, respectively. Among the selected study areas, the mean apparent seroprevalence was significantly greater in Bishoftu (35.9%), Holeta (34.2%) and Adaberga (28.6%) than in Mekelle (9.9%) and Ambo (16.2%). Among the specific seroprevalence in specific areas, BHV1 was the most common in Adaberga, with an apparent seroprevalence of 92.9%. Similarly, the seroprevalence of BVDV was the highest in Holeta, with an apparent seroprevalence of 73.3%. On the other hand, no seropositive animal to Brucella spp. or C. abortus was found in these study areas.
BVDV and BHV1 seroprevalence was higher in dairy cattle with a history of reproductive disorder in Ethiopia as compared to the seroprevalence of N. caninum and C. burnetii.
繁殖问题是一个与动物健康相关的瓶颈，制约着埃塞俄比亚等热带国家的家畜遗传改良工作。繁殖障碍的感染性病因是繁殖效率下降的一个原因。本研究旨在确定埃塞俄比亚选定地区患有繁殖障碍的奶牛中牛疱疹病毒1型（BHV1）、牛病毒性腹泻病毒（BVDV）、犬新孢子虫（N. caninum）和伯氏考克斯体（C. burnetii）的血清阳性率。总体而言，2018年10月至2019年5月期间，从有繁殖障碍病史的动物中采集了164份血清样本。使用玫瑰红平板试验和酶联免疫吸附测定法检测所采集血清中针对布鲁氏菌属、犬新孢子虫、BVDV、BHV1、伯氏考克斯体和流产嗜衣原体（C. abortus）的抗体效价。
BHV1、BVDV、犬新孢子虫和伯氏考克斯体的表观血清阳性率分别为61%、33.5%、4.9%和0.6%。在选定的研究地区中，比绍夫图（35.9%）、霍莱塔（34.2%）和阿达贝加（28.6%）的平均表观血清阳性率显著高于梅克内（9.9%）和安博（16.2%）。在特定地区的特定血清阳性率中，BHV1在阿达贝加最为常见，表观血清阳性率为92.9%。同样，BVDV的血清阳性率在霍莱塔最高，表观血清阳性率为73.3%。另一方面，在这些研究地区未发现布鲁氏菌属或流产嗜衣原体的血清阳性动物。
与犬新孢子虫和伯氏考克斯体的血清阳性率相比，埃塞俄比亚有繁殖障碍病史的奶牛中BVDV和BHV1的血清阳性率更高。
