Tamano Koichi
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan.
Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
Front Fungal Biol. 2024 Dec 16;5:1526568. doi: 10.3389/ffunb.2024.1526568. eCollection 2024.
Research on enhancing the production of lipids, particularly polyunsaturated fatty acids that are considered important for health, has focused on improvement of metabolism as well as heterologous expression of biosynthetic genes in the oleaginous fungus . To date, the productivity and production yield of free fatty acids have been enhanced by 10-fold to 90-fold via improvements in metabolism and optimization of culture conditions. Moreover, the productivity of ester-type fatty acids present in triacylglycerols could be enhanced via metabolic improvement. Culturing in a liquid medium supplemented with non-ionic surfactants could also lead to the effective release of free fatty acids from the cells. The current review highlights the advancements made in this field so far and discusses the future outlook for research on lipid production using . I hope the contents are useful for researchers in this field to consider the strategy of increasing production of various valuable metabolites as well as lipids in .
关于提高脂质产量，特别是对健康有益的多不饱和脂肪酸产量的研究，主要集中在改善代谢以及在产油真菌中对生物合成基因进行异源表达。迄今为止，通过改善代谢和优化培养条件，游离脂肪酸的生产率和产量已提高了10倍至90倍。此外，通过代谢改善可提高三酰甘油中酯型脂肪酸的生产率。在添加非离子表面活性剂的液体培养基中培养也可导致细胞中游离脂肪酸的有效释放。本综述重点介绍了该领域目前取得的进展，并讨论了利用……进行脂质生产研究的未来前景。我希望这些内容对该领域的研究人员有所帮助，以便他们考虑提高……中各种有价值代谢物以及脂质产量的策略。
