Bessalah Salma, Faraz Asim, Mustafa Ayman Balla, Hussain Syeda Maryam, Saeed Shamsaldeen Ibrahim, Liaqat Chanda, Ashraf Waqas, Iqbal Zeeshan Muhammad, Akbar Muhammad Arslan, Hammadi Mohamed
Livestock and Wildlife Laboratory, Arid Lands Institute (I.R.A), University of Gabès, Médenine, Tunisia.
Department of Livestock and Poultry Production, Bahauddin Zakariya University, Multan, Pakistan.
Open Vet J. 2024 Nov;14(11):2883-2892. doi: 10.5455/OVJ.2024.v14.i11.17. Epub 2024 Nov 30.
Many protective proteins, including lactoferrin and heavy chain antibodies, are present in camel colostrum, giving it a distinctive composition. Beyond a broad spectrum of pathogens, these proteins demonstrate antibacterial properties.
The current research assessed the prophylactic properties of camel colostrum against F17.
A microbroth dilution method was employed to assess the efficacy of camel colostrum, whereas a crystal violet assay was utilized to determine its antibiofilm potential. Extracellular deoxyribonuclease acid (eDNA) release, swarming, and swimming motilities were also examined.
Showed that camel colostrum significantly reduced . -F17 growth by 70% and above at different incubation periods (6-24 hours). The rate of cell attachment gradually decreased from approximately 40% to 24% as the concentration increased from 12.5 to 50 mg/ml. . -F17 developed a biofilm at a rate of 54.8% when exposed to 50 mg/ml of camel colostrum. In contrast, the greatest level of biofilm formation against the tested bacteria (94%) was observed at a concentration of 1.5 mg/ml. A halo zone of camel colostrum ranging from 10 to less than 30 mm at concentrations between 6 and 50 mg/ml also inhibited swimming and swarming capabilities. The treated cells yielded no eDNA.
According to these results, camel colostrum inhibits the growth of . -F17 by impeding the swarming and swimming motilities, and biofilm formation. Additionally, camel colostrum incubation with . -F17 diminishes eDNA. To evaluate the potential protective effects of camel colostrum in an animal model, additional research is recommended.
骆驼初乳中存在许多保护性蛋白质，包括乳铁蛋白和重链抗体，使其具有独特的成分。除了对多种病原体具有广泛作用外，这些蛋白质还具有抗菌特性。
当前研究评估了骆驼初乳对F17的预防特性。
采用微量肉汤稀释法评估骆驼初乳的功效，同时利用结晶紫测定法确定其抗生物膜潜力。还检测了细胞外脱氧核糖核酸（eDNA）释放、群集运动和游动运动。
表明骆驼初乳在不同孵育期（6 - 24小时）可使F17的生长显著降低70%及以上。随着浓度从12.5毫克/毫升增加到50毫克/毫升，细胞附着率从约40%逐渐降至24%。当暴露于50毫克/毫升的骆驼初乳时，F17形成生物膜的速率为54.8%。相比之下，在浓度为1.5毫克/毫升时观察到针对受试细菌的生物膜形成水平最高（94%）。浓度在6至50毫克/毫升之间的骆驼初乳形成的晕圈范围为10至小于30毫米，也抑制了游动和群集能力。处理后的细胞未产生eDNA。
根据这些结果，骆驼初乳通过阻碍群集运动、游动运动和生物膜形成来抑制F17的生长。此外，骆驼初乳与F17孵育可减少eDNA。建议开展更多研究以评估骆驼初乳在动物模型中的潜在保护作用。
