Faculty of Veterinary Medicine, National Major San Marcos University, Av. Circunvalacion 2800, San Borja, Lima, Peru.
CONOPA- Institute for Research and Development of South American Camelids, Av. Reusche M4, Pachacamac, Lima, Peru.
Trop Anim Health Prod. 2020 Nov;52(6):3917-3921. doi: 10.1007/s11250-020-02415-2. Epub 2020 Sep 29.
Neonatal domestic South American Camelid llamas and alpacas suffer from an enteric disease complex characterized by abdominal distention, lethargy, dehydration, and eventual fatal septicemia. Analysis of rectal swabs from neonatal alpacas suffering clinical diarrheas has constantly isolated Escherichia coli, mainly the EPEC and EHEC pathotypes. The present communication reports the results of molecular analysis of 226 E. coli strains from neonatal alpaca rectal swabs. The isolates were initially tested by multiple PCR, to identify E. coli virulence genes eae, bfp, Lt, Stx1, Stx2, sta, stab, and lt genes and a similar test to detect F4, F5, F6, F17, and F41fimbriae adhesin genes. Forty-two of the 226 (18.5%) isolates tested positive for at least one pathogenic gene, 25 of the 42 were classified as EPEC (3 positives for only eae and 22 for both eae and bfp) and the remaining 17 were classified as EHEC. Twenty-four (57%) of the 42 isolates tested positive to F17 adhesin while one was positive for both F6 and F17.
新生南美驼羊（羊驼）和骆马患有一种以腹部膨胀、嗜睡、脱水和最终致命败血症为特征的肠病。对患有临床腹泻的新生骆马直肠拭子的分析不断分离出大肠杆菌，主要是 EPEC 和 EHEC 血清型。本报告介绍了对 226 株来自新生骆马直肠拭子的大肠杆菌菌株的分子分析结果。这些分离株最初通过多重 PCR 进行测试，以鉴定大肠杆菌毒力基因 eae、bfp、Lt、Stx1、Stx2、sta、stab 和 lt 基因，以及类似的测试来检测 F4、F5、F6、F17 和 F41 菌毛黏附素基因。在 226 个分离株中，有 42 个（18.5%）至少检测到一个致病基因呈阳性，其中 25 个被归类为 EPEC（仅 3 个阳性为 eae，22 个阳性为 eae 和 bfp），其余 17 个被归类为 EHEC。在 42 个分离株中，有 24 个（57%）对 F17 黏附素呈阳性，而有 1 个对 F6 和 F17 均呈阳性。
