Wagner Nicole D, Prater Clay, Robbins Caleb J, Osburn Felicia S, Wang Jingyu, Jeyasingh Punidan D, Scott J Thad
Department of Biological Sciences, Oakland University, Rochester, Michigan, USA.
Department of Integrative Biology, Oklahoma State University, Stillwater, Oklahoma, USA.
Ecol Lett. 2025 Jan;28(1):e70051. doi: 10.1111/ele.70051.
Diazotrophic cyanobacteria can overcome nitrogen (N)-limitation by fixing atmospheric N; however, this increases their energetic, iron, molybdenum, and boron costs. It is unknown how current and historic N-supplies affect cyanobacterial elemental physiology beyond increasing demands for elements involved in N-fixation. Here, we examined the changes in pigment concentrations, N-storage, and the ionome (i.e., multivariate elemental composition) of the freshwater diazotroph Dolichospermum flosaquae adapted to an N-gradient for two temporal scales: 27 days and 45 months. We found short-term adaptation of Dolichospermum to low N-supply decreased pigment concentrations, N-storage, N:carbon (C), and increased boron:C, calcium:C, and magnesium:C than high N-supply adapted populations. Dolichospermum adapted to low N-supplies for 45 months had higher pigment concentrations, N-storage, and lower boron:C, calcium:C, magnesium:C, and phosphorus:C than the short-term adapted populations when grown in low N-supplies. Our results highlight the connections between the ionome and physiology, identifying the previously unrecognised roles of elements that can be used to advance physiological patterns.
固氮蓝藻可以通过固定大气中的氮来克服氮限制；然而，这增加了它们在能量、铁、钼和硼方面的成本。目前和历史上的氮供应如何影响蓝藻元素生理，除了增加对参与固氮的元素的需求外，尚不清楚。在这里，我们研究了淡水固氮生物弗氏多列藻适应氮梯度在两个时间尺度上（27天和45个月）色素浓度、氮储存和离子组（即多变量元素组成）的变化。我们发现，与适应高氮供应的群体相比，多列藻对低氮供应的短期适应降低了色素浓度、氮储存、氮：碳（C），并增加了硼：碳、钙：碳和镁：碳。当在低氮供应条件下生长时，适应低氮供应45个月的多列藻比短期适应群体具有更高的色素浓度、氮储存，以及更低的硼：碳、钙：碳、镁：碳和磷：碳。我们的结果突出了离子组与生理之间的联系，确定了可用于推进生理模式的元素以前未被认识到的作用。
