Gezginer Irmak, Chen Zhenyue, Yoshihara Hikari A I, Deán-Ben Xosé Luís, Zerbi Valerio, Razansky Daniel
Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Zurich, Switzerland.
Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich, Switzerland.
Nat Commun. 2024 Dec 30;15(1):10791. doi: 10.1038/s41467-024-54947-y.
Resting-state functional connectivity (rsFC) has been essential to elucidate the intricacy of brain organization, further revealing clinical biomarkers of neurological disorders. Although functional magnetic resonance imaging (fMRI) remains a cornerstone in the field of rsFC recordings, its interpretation is often hindered by the convoluted physiological origin of the blood-oxygen-level-dependent (BOLD) contrast affected by multiple factors. Here, we capitalize on the unique concurrent multiparametric hemodynamic recordings of a hybrid magnetic resonance optoacoustic tomography platform to comprehensively characterize rsFC in female mice. The unique blood oxygenation readings and high spatio-temporal resolution at depths provided by functional optoacoustic (fOA) imaging offer an effective means for elucidating the connection between BOLD and hemoglobin responses. Seed-based and independent component analyses reveal spatially overlapping bilateral correlations between the fMRI-BOLD readings and the multiple hemodynamic components measured with fOA but also subtle discrepancies, particularly in anti-correlations. Notably, total hemoglobin and oxygenated hemoglobin components are found to exhibit stronger correlation with BOLD than deoxygenated hemoglobin, challenging conventional assumptions on the BOLD signal origin.
静息态功能连接（rsFC）对于阐明脑组织的复杂性至关重要，进一步揭示了神经疾病的临床生物标志物。尽管功能磁共振成像（fMRI）仍然是rsFC记录领域的基石，但其解释常常受到多种因素影响的血氧水平依赖（BOLD）对比的复杂生理起源的阻碍。在此，我们利用混合磁共振光声断层扫描平台独特的同步多参数血流动力学记录，全面表征雌性小鼠的rsFC。功能光声（fOA）成像提供的独特血氧读数和深度处的高时空分辨率，为阐明BOLD与血红蛋白反应之间的联系提供了一种有效手段。基于种子点和独立成分分析揭示了fMRI-BOLD读数与fOA测量的多个血流动力学成分之间在空间上重叠的双侧相关性，但也存在细微差异，特别是在反相关性方面。值得注意的是，发现总血红蛋白和氧合血红蛋白成分与BOLD的相关性比脱氧血红蛋白更强，这对关于BOLD信号起源的传统假设提出了挑战。
