DCA基因过表达对转基因微小杜氏藻的影响。
Effects of DCA gene overexpression in transgenic Dunaliella parva.
作者信息
Ruan Lingru, Zhou Bingfeng, Liang Yanyan, Zhang Xiumin, Wu Lina, Huang Limei, Liang Shiyu, Shang Changhua
机构信息
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
出版信息
Antonie Van Leeuwenhoek. 2024 Dec 31;118(2):46. doi: 10.1007/s10482-024-02054-9.
Duplicated carbonic anhydrase was a member of carbonic anhydrase family. The ubiquitous carbonic anhydrase (CA) plays important roles in plants. However, the role of CA in Dunaliella parva remains to be further explored. In this study, a member of the CAs family was isolated from D. parva and named DpDCA. It was transferred into D. parva by genetic engineering techniques. The carotenoid, total carbohydrate, starch, protein, and oil contents of transgenic D. parva increased by 16.31%, 31.68%, 43.97%, 52.91%, and 12.32%, compared to control. Perhaps DpDCA affected photosynthesis through cis-element. Therefore, this study lays a foundation for studying the function of DpDCA gene in D. parva.
重复碳酸酐酶是碳酸酐酶家族的一员。普遍存在的碳酸酐酶（CA）在植物中发挥着重要作用。然而，CA在微小杜氏藻中的作用仍有待进一步探索。在本研究中，从微小杜氏藻中分离出一个CA家族成员，并将其命名为DpDCA。通过基因工程技术将其转入微小杜氏藻中。与对照相比，转基因微小杜氏藻的类胡萝卜素、总碳水化合物、淀粉、蛋白质和油脂含量分别增加了16.31%、31.68%、43.97%、52.91%和12.32%。也许DpDCA通过顺式元件影响光合作用。因此，本研究为研究DpDCA基因在微小杜氏藻中的功能奠定了基础。
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