Xiong Dan, Sun Tao, Liu Mengru, Wang Bo, Guan Tianzhu, Song Li, Jiao Xinan, Yang Zhenquan
College of Food Science and Engineering, Yangzhou University, 196 West Huayang Road, Yangzhou, 225127, Jiangsu, China.
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, 225009, China.
Probiotics Antimicrob Proteins. 2024 Dec 30. doi: 10.1007/s12602-024-10440-y.
Foodborne bacterial enteritis is a common clinical disease, and its incidence has risen globally. To screen for functional Bacillus strains with anti-inflammatory properties, tolerance to acid and bile salts, and antagonism against Salmonella, 22 strains of Bacillus were employed as candidate strains in this study. An inflammatory cell model was established using J774-Dual NF-κB/IRF reporter macrophages to identify anti-inflammatory Bacillus. The candidate Bacillus strains were assessed through tolerance to acid and bile salts and antibacterial assays, and their inhibitory effects on the inflammatory responses triggered by S. Enteritidis infection were investigated. The findings demonstrated that B. licheniformis B410 was successfully screened, possessing a significant anti-inflammatory effect by suppressing the NF-κB and IRF signaling pathways. B410 exhibited excellent tolerance to acid and bile salts and displayed a favorable antibacterial effect against Salmonella. Co-incubation of B410 with RAW264.7 macrophages did not influence the cell viability. B. licheniformis B410 could significantly inhibit the expression of pro-inflammatory cytokines IL-1β and TNF-α induced by LPS and promote the expression of the anti-inflammatory cytokine IL-10. Additionally, B410 could markedly inhibit the activation of NF-κB and the production of inflammatory cytokines caused by S. Enteritidis infection in macrophages. This study successfully screened a new strain of B. licheniformis B410 that simultaneously had the capabilities of anti-inflammation, acid and bile salt tolerance, and antagonism against Salmonella, providing a new approach for the screening of functional anti-inflammatory probiotics and the development of anti-inflammatory probiotic therapeutic preparations.
食源性细菌性肠炎是一种常见的临床疾病，其发病率在全球范围内呈上升趋势。为筛选具有抗炎特性、耐酸和耐胆汁盐以及对沙门氏菌有拮抗作用的功能性芽孢杆菌菌株，本研究选用了22株芽孢杆菌作为候选菌株。利用J774-双NF-κB/IRF报告基因巨噬细胞建立炎症细胞模型来鉴定抗炎芽孢杆菌。通过耐酸和耐胆汁盐试验以及抗菌试验对候选芽孢杆菌菌株进行评估，并研究它们对肠炎沙门氏菌感染引发的炎症反应的抑制作用。研究结果表明，成功筛选出地衣芽孢杆菌B410，它通过抑制NF-κB和IRF信号通路具有显著的抗炎作用。B410对酸和胆汁盐表现出优异的耐受性，对沙门氏菌具有良好的抗菌作用。B410与RAW264.7巨噬细胞共孵育不影响细胞活力。地衣芽孢杆菌B410可显著抑制脂多糖诱导的促炎细胞因子IL-1β和TNF-α的表达，并促进抗炎细胞因子IL-10的表达。此外，B410可显著抑制巨噬细胞中肠炎沙门氏菌感染引起的NF-κB激活和炎症细胞因子的产生。本研究成功筛选出一株同时具有抗炎、耐酸和耐胆汁盐以及拮抗沙门氏菌能力的新型地衣芽孢杆菌B410，为功能性抗炎益生菌的筛选和抗炎益生菌治疗制剂的开发提供了新途径。
