Foglesong P D
J Virol. 1985 Mar;53(3):822-6. doi: 10.1128/JVI.53.3.822-826.1985.
Faithful transcription of a vaccinia virus gene was accomplished in vitro by using a soluble extract prepared from vaccinia virus-infected HeLa cells. Specific transcription of the cloned vaccinia virus gene was detected by using template DNA restricted within the transcribed region. The vaccinia virus gene was not transcribed by extracts prepared from uninfected HeLa cells even with supplementation by purified vaccinia virus RNA polymerase, nor was a clone of adenovirus 2 DNA bearing the major late promoter transcribed by the extract from vaccinia virus-infected HeLa cells. Thus, infection by vaccinia virus altered cellular transcriptional specificity to favor expression of vaccinia virus genes. RNA synthesis by the infected cell extract was resistant to alpha-amanitin but strongly inhibited by beta, gamma-imido ATP and novobiocin.
通过使用从感染痘苗病毒的HeLa细胞制备的可溶性提取物，在体外实现了痘苗病毒基因的忠实转录。通过使用在转录区域内受限制的模板DNA来检测克隆的痘苗病毒基因的特异性转录。即使补充纯化的痘苗病毒RNA聚合酶，未感染的HeLa细胞制备的提取物也不会转录痘苗病毒基因，感染痘苗病毒的HeLa细胞提取物也不会转录带有主要晚期启动子的腺病毒2 DNA克隆。因此，痘苗病毒感染改变了细胞转录特异性，以利于痘苗病毒基因的表达。感染细胞提取物的RNA合成对α-鹅膏蕈碱具有抗性，但受到β，γ-亚氨基ATP和新生霉素的强烈抑制。
