Venkatesan S, Baroudy B M, Moss B
Cell. 1981 Sep;25(3):805-13. doi: 10.1016/0092-8674(81)90188-4.
Poxviruses, unlike other DNA viruses, replicate in the cytoplasm of infected cells and use their own system of transcription. Examination on one early mRNA synthesized in vivo and in vitro indicated that it has multiple closely spaced 5' and 3' ends. A remarkable 88% AT-rich 60 bp DNA sequence was found immediately upstream of the initiation of transcription sites. Although DNA sequences that bear some homology to Pribnow and Hogness boxes are present, additional recognition sequences located further upstream of procaryotic and eucaryotic initiation sites are absent. A possible initiation of translation codon occurs about 50 nucleotides from the 5' end of the message. The transcript terminates near or within a hexanucleotide CTATTC that is tandemly repeated four times. Sequences similar to those regulating termination of transcription in procaryotes or poly (A) addition in eucaryotes were not found, suggesting that poxviruses have evolved unique recognition signals.
痘病毒与其他DNA病毒不同，在受感染细胞的细胞质中复制，并使用自身的转录系统。对一种在体内和体外合成的早期mRNA的研究表明，它有多个紧密间隔的5'和3'末端。在转录起始位点上游立即发现了一个显著的富含88% AT的60 bp DNA序列。虽然存在与普里布诺盒和霍格内斯盒有一定同源性的DNA序列，但在原核生物和真核生物起始位点上游更远的位置不存在额外的识别序列。一个可能的翻译起始密码子出现在距信息5'端约50个核苷酸处。转录本在一个六核苷酸CTATTC附近或其中终止，该六核苷酸串联重复四次。未发现与原核生物转录终止或真核生物聚腺苷酸化调控序列相似的序列，这表明痘病毒已经进化出独特的识别信号。
