Yan Yunxia, Zhang Yue, Tang Xiaojun, Zhuoya Zhang, Linyu Geng, Lingyun Sun
Department of Rheumatology and Immunology, The Drum Tower Clinical Medical School of Nanjing Medical University, Nanjing, Jiangsu, China.
Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital Clinical College of Jiangsu University, Zhenjiang, China.
Int J Rheum Dis. 2025 Jan;28(1):e70040. doi: 10.1111/1756-185X.70040.
γδT cells have been implicated in the pathogenesis of autoimmune diseases. The study aims to investigate the abundance of γδT cells in MRL/lpr mice.
MRL/lpr mice were used as lupus models, while C3H/HeJ mice served as normal controls. The abundance of γδT cells in different organs was examined by flow cytometry. Plasma double-stranded DNA antibody levels, blood urea nitrogen, creatinine, and urinary protein levels were measured. Renal histopathology was observed via H&E staining. The correlations between the abundance of γδT cells and lupus manifestations were analyzed.
Compared with C3H/HeJ mice, the number of γδT cells and Vγ6γδT cell subset in the peripheral blood of MRL/lpr mice was significantly reduced. However, in the kidney, the number of γδT cells and Vγ6γδT cell subset was significantly increased. Additionally, the number of Vγ6γδT cells in the kidney was positively correlated with the urinary protein level. The number of IFN-γVγ6γδT cells in the kidney was positively correlated with urinary protein level.
In MRL/lpr mice, it is likely that peripheral γδT cells, especially the Vγ6 subset, infiltrate the kidney and secrete IFN-γ, which contributes to the development of lupus nephritis.
γδT细胞与自身免疫性疾病的发病机制有关。本研究旨在调查MRL/lpr小鼠中γδT细胞的丰度。
将MRL/lpr小鼠用作狼疮模型，而C3H/HeJ小鼠作为正常对照。通过流式细胞术检测不同器官中γδT细胞的丰度。测量血浆双链DNA抗体水平、血尿素氮、肌酐和尿蛋白水平。通过苏木精-伊红染色观察肾脏组织病理学。分析γδT细胞丰度与狼疮表现之间的相关性。
与C3H/HeJ小鼠相比，MRL/lpr小鼠外周血中γδT细胞和Vγ6γδT细胞亚群的数量显著减少。然而，在肾脏中，γδT细胞和Vγ6γδT细胞亚群的数量显著增加。此外，肾脏中Vγ6γδT细胞的数量与尿蛋白水平呈正相关。肾脏中IFN-γVγ6γδT细胞的数量与尿蛋白水平呈正相关。
在MRL/lpr小鼠中，外周γδT细胞，尤其是Vγ6亚群，可能浸润肾脏并分泌IFN-γ，这有助于狼疮性肾炎的发展。
