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使用CRISPR/Cas13a和侧向流动分析法快速检测泛禽流感病毒以及禽流感病毒的H5、H7、H9亚型

Rapid detection of Pan-Avian Influenza Virus and H5, H7, H9 subtypes of Avian Influenza Virus using CRISPR/Cas13a and lateral flow assay.

作者信息

Yang Yujia, Yang Zhiyi, Zhang Xinkui, Niu Beibei, Huang Qiuhong, Li Yan, Yin Huifang, Zhang Xianpeng, Liao Ming, Jia Weixin

机构信息

Guangdong Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, National Avian Influenza Para-Reference Laboratory (Guangzhou), College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.

Engineering Research Center for the Prevention and Control of Animal Original Zoonosis of Fujian Province University, College of Life Science, Longyan University, Longyan, 364012, China.

出版信息

Poult Sci. 2025 Feb;104(2):104745. doi: 10.1016/j.psj.2024.104745. Epub 2024 Dec 28.

DOI:10.1016/j.psj.2024.104745
PMID:39740498
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11750554/
Abstract

Avian Influenza Virus (AIV) has been prevalent worldwide in recent years, resulting in substantial economic losses in the poultry industry. More importantly, AIV is capable of cross-species transmission among mammals, posing a dormant yet considerable threat to human health and safety. In this study, two rapid detection methods for AIV based on the CRISPR-Cas13a were developed. These methods can identify AIV through the M gene and differentiate the H5, H7, and H9 subtypes via the HA gene. The first method utilizes RT-RAA isothermal amplification of the target sequence in combination with the "collateral effect" of the Cas13a protein. The results are measured using a real-time quantitative PCR instrument, with a Limit of Detection (LOD) as low as 1 copy/μL. The second method combines RT-RAA with Cas13a and a lateral flow assay, allowing results to be visually observed with the naked eye, with a LOD of 10 copies/μL. Both methods demonstrated specificity and sensitivity comparable to or exceeding that of qRT-PCR, suggesting strong potential for clinical application.

摘要

近年来，禽流感病毒（AIV）在全球范围内广泛流行，给家禽业造成了巨大的经济损失。更重要的是，AIV能够在哺乳动物之间进行跨物种传播，对人类健康和安全构成潜在但相当大的威胁。在本研究中，开发了两种基于CRISPR-Cas13a的AIV快速检测方法。这些方法可以通过M基因鉴定AIV，并通过HA基因区分H5、H7和H9亚型。第一种方法利用靶序列的RT-RAA等温扩增结合Cas13a蛋白的“附带效应”。使用实时定量PCR仪测量结果，检测限（LOD）低至1拷贝/μL。第二种方法将RT-RAA与Cas13a和侧向流动分析相结合，使结果能够用肉眼直观观察，LOD为10拷贝/μL。两种方法都显示出与qRT-PCR相当或超过qRT-PCR的特异性和灵敏度，表明具有很强的临床应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/81bdada05a7b/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/73cacb54c2e8/gr1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/f1a582b91cf8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/25211f51c7e2/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/ca0a07753e3a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/8273e799b272/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/80cc8aefb199/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/957bc3afdaed/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/d4f4b0b823e6/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/891ba92dc868/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/81bdada05a7b/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/73cacb54c2e8/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/4ac862807439/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/c5de67a6a812/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/f1a582b91cf8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/25211f51c7e2/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/ca0a07753e3a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/8273e799b272/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/80cc8aefb199/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/957bc3afdaed/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/d4f4b0b823e6/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/891ba92dc868/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4d3/11750554/81bdada05a7b/gr12.jpg

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本文引用的文献

1
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Anal Methods. 2024 Jul 11;16(27):4496-4515. doi: 10.1039/d4ay00508b.
2
Recent Changes in Patterns of Mammal Infection with Highly Pathogenic Avian Influenza A(H5N1) Virus Worldwide.全球高致病性禽流感 A(H5N1)病毒感染哺乳动物模式的最新变化。
Emerg Infect Dis. 2024 Mar;30(3):444-452. doi: 10.3201/eid3003.231098.
3
Advances in Detection Techniques for the H5N1 Avian Influenza Virus.
H5N1 禽流感病毒检测技术的进展。
Int J Mol Sci. 2023 Dec 5;24(24):17157. doi: 10.3390/ijms242417157.
4
Rapid detection of H5 subtype avian influenza virus using CRISPR Cas13a based-lateral flow dipstick.基于CRISPR Cas13a的侧流试纸条快速检测H5亚型禽流感病毒
Front Microbiol. 2023 Nov 29;14:1283210. doi: 10.3389/fmicb.2023.1283210. eCollection 2023.
5
Rapid and sensitive Cas13a/Cas12a-based one-pot dual-target strategy to detect monkeypox virus and its co-infected viruses.基于Cas13a/Cas12a的快速灵敏一锅法双靶标策略检测猴痘病毒及其共感染病毒
Sci Bull (Beijing). 2023 Dec 30;68(24):3142-3148. doi: 10.1016/j.scib.2023.11.023. Epub 2023 Nov 11.
6
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Front Microbiol. 2023 Oct 11;14:1288951. doi: 10.3389/fmicb.2023.1288951. eCollection 2023.
7
One-tube RPA-CRISPR Cas12a/Cas13a rapid detection of methicillin-resistant Staphylococcus aureus.一管式 RPA-CRISPR Cas12a/Cas13a 快速检测耐甲氧西林金黄色葡萄球菌。
Anal Chim Acta. 2023 Oct 16;1278:341757. doi: 10.1016/j.aca.2023.341757. Epub 2023 Aug 26.
8
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Emerg Infect Dis. 2023 Jul;29(7):1367-1375. doi: 10.3201/eid2907.221149.
9
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10
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Genes Dis. 2022 Dec 27;10(6):2414-24. doi: 10.1016/j.gendis.2022.11.016.