Yamaki J, Yamamoto T, Okada H
J Steroid Biochem. 1985 Jan;22(1):63-6. doi: 10.1016/0022-4731(85)90142-6.
The ability of human uterine endometrium to aromatize androstenedione to estrogens was investigated using 10 normal and neoplastic tissues. Normal and neoplastic endometrial homogenates were incubated with [6,7-3H]androstenedione (A) and NADPH. Estrone (E1) and estradiol (E2) were subsequently isolated in amounts ranging from 0-17600 fmol/h/g and 0-377 fmol/h/g, respectively, from the incubates after purifications by using Bio-Rad AG1-X2 column, thin layer chromatographies and co-crystallization. The conversion of A to E1 and E2 was significantly higher in neoplastic tissues.
利用10份正常组织和肿瘤组织研究了人子宫内膜将雄烯二酮芳香化转化为雌激素的能力。将正常和肿瘤子宫内膜匀浆与[6,7-³H]雄烯二酮（A）和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）一起孵育。随后，通过使用伯乐AG1-X2柱、薄层色谱法和共结晶法进行纯化后，从孵育物中分别分离出雌酮（E1）和雌二醇（E2），其量分别在0 - 17600飞摩尔/小时/克和0 - 377飞摩尔/小时/克范围内。在肿瘤组织中，A向E1和E2的转化显著更高。
