Werber A H, Fink G D
J Pharmacol Methods. 1985 Feb;13(1):67-82. doi: 10.1016/0160-5402(85)90069-5.
Changes in vascular reactivity are thought to play an important role in the maintenance and/or pathogenesis of various disorders of the cardiovascular system including hypertension. In some animal models, results of studies examining reactivity of the hindquarters vasculature using different techniques are conflicting. The purpose of this study was to validate a new method for measuring vascular reactivity of the hindquarters: a method based on a modification of a technique used previously for renal autoperfusion. We tested the method in spontaneously hypertensive rats (SHR) in order to allow comparison of our results to those obtained previously using other techniques. We found that reactivity of the SHR hindquarters to norepinephrine was normal (compared to that of normotensive Wistar-Kyoto control rats, WKY), while reactivity to nerve stimulation was increased (when results were expressed as an absolute change in resistance). Dilator reactivity to acetylcholine and sympathetic nerve crush was greater in SHR. Resistance of the hindquarters at maximal dilation was 25% higher in SHR. Except for the resistance at maximal dilation, these results contrast with those obtained in earlier studies using an artificial pump to perfuse SHR hindquarters with blood or artificial media. However, the results agree with those reported in conscious, adult SHR. We conclude that: 1) our autoperfusion technique allows comparatively simple vascular reactivity testing to be performed in blood-perfused hindquarters of the rat, and 2) reactivity of the SHR hindquarters to dilator stimuli may be greater than in WKY rats.
血管反应性的变化被认为在包括高血压在内的各种心血管系统疾病的维持和/或发病机制中起重要作用。在一些动物模型中，使用不同技术检测后肢血管系统反应性的研究结果相互矛盾。本研究的目的是验证一种测量后肢血管反应性的新方法：一种基于对先前用于肾脏自身灌注的技术进行改进的方法。我们在自发性高血压大鼠（SHR）中测试了该方法，以便将我们的结果与先前使用其他技术获得的结果进行比较。我们发现，SHR后肢对去甲肾上腺素的反应性正常（与正常血压的Wistar-Kyoto对照大鼠WKY相比），而对神经刺激的反应性增加（当结果以阻力的绝对变化表示时）。SHR中对乙酰胆碱和交感神经挤压的扩张反应性更大。SHR在最大扩张时后肢的阻力高25%。除最大扩张时的阻力外，这些结果与早期使用人工泵用血液或人工介质灌注SHR后肢的研究结果相反。然而，这些结果与清醒成年SHR中报道的结果一致。我们得出以下结论：1）我们的自身灌注技术允许在大鼠血液灌注的后肢中进行相对简单的血管反应性测试，2）SHR后肢对扩张刺激的反应性可能大于WKY大鼠。
