Connors S, Meyer F J, Sundheimer D W, Sipes I G, Brendel K
J Pharmacol Methods. 1985 Feb;13(1):83-94. doi: 10.1016/0160-5402(85)90070-1.
An all-glass apparatus for perfusing isolated rat livers was constructed for use in studies of the metabolism by, or action on, the liver of lipophilic substrates. Other designs, which generally include plastic connections or cannulae, can have unnecessarily complicated kinetics because of adsorption of the agent to the plastic parts. Two versions of an all-glass perfusion apparatus are described in detail. Both instruments operate on the same basic principle, but differ in size, total recirculating volume, pump rate, perfusion head pressures, maximum perfusate flow rate, maximum linear velocity in the perfusion head, and oxygenation efficiency. Although the maximum protein handling capacity differs for the two apparatuses, this capacity is so limited that it disallows the use of albumin in the perfusate.
构建了一种用于灌注离体大鼠肝脏的全玻璃装置，用于研究亲脂性底物在肝脏中的代谢或对肝脏的作用。其他设计通常包括塑料连接件或插管，由于试剂吸附到塑料部件上，可能具有不必要的复杂动力学。详细描述了全玻璃灌注装置的两个版本。两种仪器的工作原理相同，但在尺寸、总循环体积、泵速、灌注头压力、最大灌注液流速、灌注头中的最大线速度和氧合效率方面有所不同。尽管两种装置的最大蛋白质处理能力不同，但这种能力非常有限，以至于不允许在灌注液中使用白蛋白。
