Huang Luan, Zhou Xin, Chang Qingyun, Zhang Shuaihua, Wang Zhi, Wang Chun, Zang Xiaohuan
Department of Chemistry, College of Science, Hebei Agricultural University, Baoding 071001, China.
College of Science and Technology, Hebei Agricultural University, Cangzhou, Hebei 061000, China.
Food Chem. 2025 Apr 1;470:142703. doi: 10.1016/j.foodchem.2024.142703. Epub 2024 Dec 29.
Sulfonamide antibiotics have a broad spectrum of antibacterial action and are widely used, but their overuse poses a threat to human health. In this study, a three-dimensional conjugated microporous polymer, which was designated as TPM-CMP, was synthesized via Friedel-Crafts reaction by using tetraphenylmethane (TPM) and biphenyl dichlorobenzene as monomers, and it was utilized as an adsorbent in solid-phase extraction (SPE) of sulfonamides. The TPM-CMP demonstrated high extraction efficiency for sulfonamides due to π-stacking interactions, hydrophobic forces, and pore-filling effects. Consequently, the SPE was combined with liquid chromatography to detect sulfonamides in milk samples. For this method established in this study, a good linear response was observed in the range of 3.3-300.0 ng g, with a detection limit (S/N = 3) between 1.0 and 1.5 ng g. The method recoveries of four sulfonamide in milk samples ranged from 83.4 % to 117 %, with relative standard deviations below 10 %.
磺胺类抗生素具有广谱抗菌作用，被广泛使用，但其过度使用对人类健康构成威胁。在本研究中，通过傅克反应，以四苯基甲烷（TPM）和联苯二氯苯为单体，合成了一种三维共轭微孔聚合物，命名为TPM-CMP，并将其用作磺胺类药物固相萃取（SPE）中的吸附剂。由于π-堆积相互作用、疏水力和孔填充效应，TPM-CMP对磺胺类药物表现出高萃取效率。因此，将固相萃取与液相色谱相结合用于检测牛奶样品中的磺胺类药物。对于本研究建立的该方法，在3.3-300.0 ng g范围内观察到良好的线性响应，检测限（S/N = 3）在1.0至1.5 ng g之间。牛奶样品中四种磺胺类药物的方法回收率在83.4%至117%之间，相对标准偏差低于10%。
