Du Jiahui, Du Le, Guo Jiushi, Sun Tao, Liu Linze, Yun Xueyan, Dong Tungalag
College of Food Science and Engineering, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, Inner Mongolia 010018, China.
College of Food Science and Engineering, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, Inner Mongolia 010018, China.
Int J Biol Macromol. 2025 Mar;294:139376. doi: 10.1016/j.ijbiomac.2024.139376. Epub 2024 Dec 30.
Chilled beef is a perishable food product. Due to the broad-spectrum antimicrobial properties of chitosan, and the biodegradability of PLLA, chitosan and PLLA are introduced double bonding groups to obtain Chitosan-graft-Glycidyl methacrylate (Cs-g-GMA) and Poly(L-lactic acid)-co-poly(butylene itaconate) (PLBI), respectively. Cs-g-GMA is then grafted onto the surface of PLBI films by UV curing to produce PLBI/Cs-g-GMA films with non-migratory, broad-spectrum antimicrobial, biodegradable, and possess good oxygen barrier properties, compared to current antimicrobial films. Antimicrobial tests in vitro confirmed that PLBI/Cs-g-GMA film could effectively inhibit the growth and reproduction of P. fluorescens, S. aureus, and E. coli. The PLBI/Cs-g-GMA films were further used for the packaging of chilled beef during 15 days of refrigerated storage. The microbiological (total viable counts (TVC), microbial diversity analysis), physicochemical (pH, total volatile basic nitrogen (TVB-N), drip loss, color) and sensory of beef were measured to evaluate the preservation effect. The results showed that PLBI/Cs-g-GMA films can significantly inhibit Pseudomonas and Aeromonas at the phylum and genus levels, respectively. Based on the good results of the preservation index, PLBI/Cs-g-GMA film could delay the quality deterioration of chilled beef by 3 to 9 days, suggesting that its considerable prospects for maintaining the storage quality of chilled beef.
冷藏牛肉是一种易腐食品。由于壳聚糖具有广谱抗菌特性以及聚乳酸（PLLA）具有生物可降解性，分别将壳聚糖和PLLA引入双键基团，得到壳聚糖接枝甲基丙烯酸缩水甘油酯（Cs-g-GMA）和聚（L-乳酸）-共-聚（衣康酸丁二酯）（PLBI）。然后通过紫外线固化将Cs-g-GMA接枝到PLBI薄膜表面，以制备出与目前的抗菌薄膜相比具有非迁移性、广谱抗菌、可生物降解且具有良好氧气阻隔性能的PLBI/Cs-g-GMA薄膜。体外抗菌测试证实，PLBI/Cs-g-GMA薄膜能够有效抑制荧光假单胞菌、金黄色葡萄球菌和大肠杆菌的生长繁殖。PLBI/Cs-g-GMA薄膜进一步用于冷藏牛肉在15天冷藏储存期间的包装。对牛肉的微生物指标（总活菌数（TVC）、微生物多样性分析）、理化指标（pH值、总挥发性盐基氮（TVB-N）、滴水损失、色泽）和感官指标进行测定，以评估保鲜效果。结果表明，PLBI/Cs-g-GMA薄膜分别在门水平和属水平上能显著抑制假单胞菌属和气单胞菌属。基于良好的保鲜指标结果，PLBI/Cs-g-GMA薄膜可将冷藏牛肉的品质劣化延缓3至9天，表明其在保持冷藏牛肉储存品质方面具有可观的前景。
