[Application Analysis of Screening for Thalassemia in the Population of Childbearing Age in Quanzhou].

OBJECTIVE

To analyze the application value of MCV, MCH and HbA in screening for thalassemia in the population of childbearing age in Quanzhou area, and to determine the optimal screening cut-off value of relevant indicators in this area.

METHODS

2 725 couples of childbearing age were included in the study and underwent routine blood test, capillary hemoglobin electrophoresis, and α and β thalassemia gene test. Statistical methods were used to analyze the distribution of thalassemia genotypes, and compare the performance of MCV, MCH, and HbA in screening various types of thalassemia. According to the ROC curve, the best cut-off values of MCV, MCH and HbA in screening for thalassemia in this area were determined.

RESULTS

In this study, a total of 1 801 thalassemia carriers were detected, including 1 341 cases of α-thalassemia, 420 cases of β-thalassemia, and 40 cases of αβ compound thalassemia. The most common genotypes of α-thalassemia and β-thalassemia were -- /αα and β /β , respectively. ROC curves were drawn to evaluate the performance of MCV, MCH and HbA in screening for α-thalassemia, mild β-thalassemia, αβ compound thalassemia, silent α-thalassemia, mild α-thalassemia, and intermediate α-thalassemia. The maximum areas under the curves (AUC) were 0.747, 0.865, 0.724, 0.486, 0.812, 0.841; 0.747, 0.846, 0.703, 0.479, 0.796, 0.903; 0.613, 0.980, 0.909, 0.465, 0.674, 0.996, respectively; and the best cut-off values corresponding to the three screening indicators were 76.15fl, 71.95fl, 77.35fl, 86.15fl, 75.41fl, 61.15fl; 24.35pg, 21.51pg, 25.45pg, 28.65pg, 24.01pg, 20.51pg; 2.45%, 3.05%, 3.55%, 3.25%, 2.45%, 1.65%, respectively.

CONCLUSION

The levels of MCV, MCH and HbA are correlated with the phenotype of thalassemia, and the detection of these indicators is of great significance for the prevention and control of thalassaemia.