Jiang N, Zhu X T, Liu Y
Central Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, Beijing100081, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2025 Jan 9;60(1):75-80. doi: 10.3760/cma.j.cn112144-20240927-00363.
Tooth development is a complex process of the orderly interaction between epithelium originating from the ectoderm and mesenchyme derived from cranial neural crest cells, which not only depends on cell genes regulatory network but also involves crosstalk between cells and their surrounding environment. Even within the same type of cellular populations, obvious heterogeneity may be observed. Single-cell RNA sequencing is a novel technology aimed at sequencing the transcriptome of individual cell. As a critical bridge linking genetic information from the genome to the functional proteome, transcriptome analysis at the single-cell level enables a deeper understanding of cellular heterogeneity and organ developmental trajectories. This approach provides a powerful tool for elucidating complex biological processes and identifying potential therapeutic targets. Here, we summarize the latest advances in employing single-cell RNA sequencing analysis to the process of tooth development, which will help provide insights into a better understanding of prosthodontics and regeneration.
牙齿发育是一个复杂的过程，涉及起源于外胚层的上皮组织与源自颅神经嵴细胞的间充质之间的有序相互作用，这不仅取决于细胞基因调控网络，还涉及细胞与其周围环境之间的相互作用。即使在同一类型的细胞群体中，也可能观察到明显的异质性。单细胞RNA测序是一种旨在对单个细胞的转录组进行测序的新技术。作为连接基因组遗传信息与功能蛋白质组的关键桥梁，单细胞水平的转录组分析能够更深入地了解细胞异质性和器官发育轨迹。这种方法为阐明复杂的生物学过程和识别潜在的治疗靶点提供了强大的工具。在此，我们总结了将单细胞RNA测序分析应用于牙齿发育过程的最新进展，这将有助于为更好地理解口腔修复学和再生提供见解。
