Nowicka Joanna, Antolova Daniela, Lass Anna, Biernat Beata, Baranowicz Karolina, Goll Aleksander, Krupinska Martyna, Ferra Bartlomiej, Strachecka Aneta, Behnke Jerzy M, Bajer Anna, Grzybek Maciej
Medical University of Gdańsk, Poland.
Institute of Parasitology SAS, Košice, Slovak Republic.
Ann Agric Environ Med. 2024 Dec 22;31(4):626-630. doi: 10.26444/aaem/184723. Epub 2024 May 9.
Rodents are recognized as reservoirs for , playing a crucial role in maintaining the parasite's presence in the environment. Biomonitoring was conducted to assess the role of sylvatic rodents in maintaining , and to analyse the prevalence and seroprevalence of the parasite in seven wild rodent species. Rodents were collected in an open grassland study site located in northeastern Poland, and dissected. Brain, spleen, blood and serum samples were collected. Molecular (PCR assay, nested-PCR assay) and serological (ELISA and agglutination tests) methods were applied to indicate the best approach for application in the biomonitoring of in small mammals. Samples were screened from 68 individuals using PCR assays but no DNA were found. The agglutination test showed no signal. Antibodies against were found in 5 sera samples out of 56 analysed (seroprevalence = 8.9% [4.4-16.8]). The results confirmed that rodents participate in the life cycle of as reservoirs of this parasite in the sylvatic environment. However, for effective bio-monitoring of in small mammals, the results suggest a preference for utilizing ELISA tests to detect antigens, as opposed to relying solely on molecular methods.
啮齿动物被认为是[寄生虫名称]的宿主，在维持该寄生虫在环境中的存在方面发挥着关键作用。开展了生物监测，以评估野生啮齿动物在维持[寄生虫名称]方面的作用，并分析七种野生啮齿动物物种中该寄生虫的流行率和血清阳性率。在波兰东北部一个开阔草地研究地点收集啮齿动物并进行解剖。采集脑、脾、血液和血清样本。应用分子方法（聚合酶链反应检测、巢式聚合酶链反应检测）和血清学方法（酶联免疫吸附测定和凝集试验）来确定在小型哺乳动物[寄生虫名称]生物监测中应用的最佳方法。使用聚合酶链反应检测对68只个体的样本进行筛查，但未发现[寄生虫名称]DNA。凝集试验未显示信号。在56份分析血清样本中的5份中发现了针对[寄生虫名称]的抗体（血清阳性率 = 8.9% [4.4 - 16.8]）。结果证实，啮齿动物作为该寄生虫在野生环境中的宿主参与了[寄生虫名称]的生命周期。然而，对于小型哺乳动物中[寄生虫名称]的有效生物监测，结果表明更倾向于利用酶联免疫吸附测定试验来检测[寄生虫名称]抗原，而不是仅依赖分子方法。
