Matic Sanja, Milovanovic Dragan, Mijailovic Zeljko, Djurdjevic Predrag, Stefanovic Srdjan, Todorovic Danijela, Vitosevic Katarina, Canovic Vanja, Popovic Suzana, Dimitrijevic Nevena Milivojevic, Zivanovic Marko, Seklic Dragana, Aleksic Sanja, Djordjevic Nemanja, Vukic Milena, Vukovic Nenad, Filipovic Nenad, Baskic Dejan, Djordjevic Natasa
Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Department of Pharmacology and Toxicology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Front Med (Lausanne). 2024 Dec 18;11:1493815. doi: 10.3389/fmed.2024.1493815. eCollection 2024.
ACE2 and TMPRSS2 represent the major gateways for SARS-CoV-2 cell entry. The presence of functional ACE2 and TMPRSS2 genetic polymorphisms that affect gene expression may affect the risk of severe form of COVID-19 and its fatal outcome.
This observational study enrolled 178 hospitalized patients diagnosed with SARS-CoV-2 infection at the University Clinical Centre of Kragujevac, Serbia. Demographic, clinical, and laboratory data were gathered at admission. Genotyping for single nucleotide polymorphisms of (rs2106809 and rs2074192) and (rs2070788 and rs4818239) was performed using the Real-Time PCR method with TaqMan assays.
Controlling for other factors of influence, such as CCI, N/L ratio, LDH level, and pO, we showed that females with rs2070788 A/A genotype were less likely to develop severe COVID-19 (odds ratio [OR] [95% confidence interval (95% CI)]: 0.030 [0.001; 0.862]). Additionally, the likelihood of dying of SARS-CoV-2 infection was lower in female carriers of at least one rs2106809 C allele (OR [95% CI]: 0.004 [0.000; 0.981]).
Our findings indicate rs2070788 and rs2106809 polymorphisms as independent predictors of severity and outcome of COVID-19 in females.
血管紧张素转换酶2（ACE2）和跨膜丝氨酸蛋白酶2（TMPRSS2）是严重急性呼吸综合征冠状病毒2（SARS-CoV-2）进入细胞的主要途径。影响基因表达的功能性ACE2和TMPRSS2基因多态性的存在可能会影响重症新型冠状病毒肺炎（COVID-19）的风险及其致命结局。
本观察性研究纳入了塞尔维亚克拉古耶瓦茨大学临床中心178例被诊断为SARS-CoV-2感染的住院患者。入院时收集人口统计学、临床和实验室数据。使用TaqMan分析法的实时聚合酶链反应（PCR）方法对ACE2（rs2106809和rs2074192）和TMPRSS2（rs2070788和rs4818239）的单核苷酸多态性进行基因分型。
在控制其他影响因素，如Charlson合并症指数（CCI）、中性粒细胞/淋巴细胞比值（N/L）、乳酸脱氢酶（LDH）水平和动脉血氧分压（pO）后，我们发现携带TMPRSS2 rs2070788 A/A基因型的女性发生重症COVID-19的可能性较小（优势比[OR][95%置信区间（95%CI）]：0.030[0.001；0.862]）。此外，至少携带一个ACE2 rs2106809 C等位基因的女性死于SARS-CoV-2感染的可能性较低（OR[95%CI]：0.004[（0.000；0.9"
我们的研究结果表明，TMPRSS2 rs2070788和ACE2 rs2106809多态性是女性COVID-19严重程度和结局的独立预测因素。 81]）。
