Zayani Zoofa, Hooshmandi Etrat, Borhani-Haghighi Afshin, Rahimi Mousa, Ostovan Vahid Reza, Fadakar Nima, Tabrizi Reza, Bayat Mahnaz, Hojati Seyedeh Shamim, Gharbi Negin, Mahmoudinasab Hamideh
Department of Biology, Islamic Azad University, Zarghan Branch, Zarghan, Iran.
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Curr J Neurol. 2024 Apr 3;23(2):117-123. doi: 10.18502/cjn.v23i2.16840.
Long non-coding ribonucleic acids (lncRNAs) have been implicated as possible circulating stroke indicators. This study focused on the expression status of antisense non-coding ribonucleic acid in the INK4 locus (ANRIL) and myocardial infarction associated transcript (MIAT) in patients with cerebral venous thrombosis (CVT). In this study, fifty patients with CVT and one hundred age/gender-matched individuals as controls were included. The circulating levels of ANRIL and MIAT in the first 24 hours after admission were evaluated using the quantitative real-time polymerase chain reaction (RT-PCR) method. We compared the expression levels of ANRIL and MIAT between patients and controls using the independent two-sample t-test. Subgroup analysis was used to investigate the association of lncRNAs with clinical characteristics in patients with CVT. Receiver operating characteristic (ROC) curve analyses were conducted to evaluate the diagnostic value of two lncRNAs in patient assessment. The relative expression of lncRNAs ANRIL and MIAT significantly decreased in patients compared to the control. ANRIL and MIAT were shown as potential markers for discriminating patients with CVT from the healthy controls with an area under the curve (AUC) of 0.98 and 0.99, respectively. For the first time, we found down-regulation and diagnostic potential of lncRNAs-ANRIL and MIAT in the blood of patients with CVT.
长链非编码核糖核酸（lncRNAs）被认为可能是循环性中风的指标。本研究聚焦于脑静脉血栓形成（CVT）患者中INK4基因座反义非编码核糖核酸（ANRIL）和心肌梗死相关转录本（MIAT）的表达状况。在本研究中，纳入了50例CVT患者和100例年龄及性别匹配的个体作为对照。采用定量实时聚合酶链反应（RT-PCR）方法评估入院后最初24小时内ANRIL和MIAT的循环水平。我们使用独立两样本t检验比较患者和对照之间ANRIL和MIAT的表达水平。亚组分析用于研究lncRNAs与CVT患者临床特征的关联。进行受试者工作特征（ROC）曲线分析以评估两种lncRNAs在患者评估中的诊断价值。与对照组相比，患者中lncRNAs ANRIL和MIAT的相对表达显著降低。ANRIL和MIAT分别显示为区分CVT患者与健康对照的潜在标志物，曲线下面积（AUC）分别为0.98和0.99。我们首次发现CVT患者血液中lncRNAs-ANRIL和MIAT的下调及诊断潜力。
