Department of Pediatrics, Xingtai People's Hospital, Xingtai, China.
Department of Gastroenterology, Minmetals Hanxing Staff General Hospital, Handan, China.
Clin Res Hepatol Gastroenterol. 2022 Apr;46(4):101895. doi: 10.1016/j.clinre.2022.101895. Epub 2022 Feb 25.
Long non-coding antisense RNAs in the INK4 locus (lnc-ANRIL) have been reported to be involved in inflammation and immunity. However, few studies have reported its clinical application in pediatric inflammatory bowel disease (IBD). Therefore, we conducted this study to investigate the correlation between lnc-ANRIL expression and disease risk, inflammation, and activity in pediatric IBD patients.
Pediatric patients with Crohn's disease (CD; n = 40), ulcerative colitis (UC; n = 40), and controls (n = 20) were recruited. For all pediatric IBD patients, lnc-ANRIL expression in peripheral blood mononuclear cells and serum inflammatory cytokine levels were measured by RT-qPCR and ELISA, respectively. For the controls, lnc-ANRIL expression was also measured.
Lnc-ANRIL levels were lower in CD (P = 0.002) and UC (P = 0.001) patients compared with the controls; negatively correlated with C-reactive protein levels (P<0.01), erythrocyte sedimentation rate (P<0.01), disease activity (P<0.05), and severity (P<0.05) in CD and UC patients; and inversely associated with tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-17, and IL-23 levels in both CD and UC patients (all P<0.01). Further subgroup analyses revealed that the association between lnc-ANRIL and inflammatory cytokines and disease activity was more remarkable in pediatric patients with moderate or severe IBD.
Lnc-ANRIL may serve as a potential marker for evaluating disease risk and monitoring disease activity in pediatric IBD patients.
INK4 基因座上的长非编码反义 RNA（lnc-ANRIL）已被报道参与炎症和免疫反应。然而，很少有研究报道其在儿科炎症性肠病（IBD）中的临床应用。因此，我们进行了这项研究，旨在探讨 lnc-ANRIL 表达与儿科 IBD 患者的疾病风险、炎症和活动之间的相关性。
招募了 40 例克罗恩病（CD）患者、40 例溃疡性结肠炎（UC）患者和 20 例对照者。通过 RT-qPCR 和 ELISA 分别检测外周血单个核细胞中的 lnc-ANRIL 表达和血清炎症细胞因子水平。对于对照组，也测量了 lnc-ANRIL 的表达。
与对照组相比，CD（P=0.002）和 UC（P=0.001）患者的 lnc-ANRIL 水平较低；与 CD 和 UC 患者的 C 反应蛋白水平（P<0.01）、红细胞沉降率（P<0.01）、疾病活动度（P<0.05）和严重程度（P<0.05）呈负相关；与 CD 和 UC 患者的肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-17 和 IL-23 水平呈负相关（均 P<0.01）。进一步的亚组分析表明，lnc-ANRIL 与炎症细胞因子和疾病活动度之间的关联在儿科中或重度 IBD 患者中更为显著。
lnc-ANRIL 可能作为评估儿科 IBD 患者疾病风险和监测疾病活动的潜在标志物。
