Pınarlık Fatihan, Babuççu Gizem, Albayrak Özgür, Doğan Özlem, Keske Şiran, Beşli Yeşim, Can Füsun, Ergönül Önder
Koç University Isbank Center for Infectious Diseases (KUISCID), İstanbul, Türkiye.
Koç University Graduate School of Health Sciences, İstanbul, Türkiye.
Infect Dis Clin Microbiol. 2024 Dec 19;6(4):258-267. doi: 10.36519/idcm.2024.367. eCollection 2024 Dec.
are clinically relevant for severity prediction and treatment of COVID-19 caused by SARS-CoV-2. We aimed to demonstrate the potential cytokines for severity prediction in the five days after symptom onset and describe the importance of serum cytokine levels for patients with different disease severity.
Hospitalized COVID-19 patients and healthy control participants were recruited, and serial sera were collected from COVID-19 patients. Thirteen cytokines, including interleukin (IL) 1β, interferon (IFN) α2, IFN- γ, tumor necrosis factor (TNF) α, monocyte chemoattractant protein (MCP-1/CCL2), IL-6, IL-8 (CXCL8), IL-10, IL-12p70, IL-17A, IL-18, IL-23, and IL-33, were studied by bead-based multiplex assay by flow cytometry. Data regarding routine laboratory test results (leucocyte count, neutrophil count, lymphocyte count, platelet count, hemoglobin, liver transaminases, C-reactive protein [CRP], procalcitonin, and creatinine) were collected.
We demonstrated that COVID-19 patients had elevated serum levels of IFN-α2, TNF-α, MCP-1/CCL2, IL-6, IL-8, IL-18, IL-33 compared to healthy participants. Elevated levels of CRP and decreased lymphocyte count were observed in the critical disease group. Longitudinal analysis revealed a statistically significant increase in IL-6, IL-18, and MCP-1 serum levels of critical patients compared to healthy controls.
MCP-1, IL-6, and IL-18 were found to be the best predictors of critical COVID-19 disease, and MCP-1 has the highest level of predictive performance.
对于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的新型冠状病毒肺炎（COVID-19）的严重程度预测和治疗具有临床相关性。我们旨在证明症状出现后五天内用于严重程度预测的潜在细胞因子，并描述血清细胞因子水平对于不同疾病严重程度患者的重要性。
招募住院的COVID-19患者和健康对照参与者，并从COVID-19患者中采集系列血清。通过基于微珠的多重检测结合流式细胞术研究了13种细胞因子，包括白细胞介素（IL）-1β、干扰素（IFN）-α2、IFN-γ、肿瘤坏死因子（TNF）-α、单核细胞趋化蛋白（MCP-1/CCL2）、IL-6、IL-8（CXCL8）、IL-10、IL-12p70、IL-17A、IL-18、IL-23和IL-33。收集有关常规实验室检查结果（白细胞计数、中性粒细胞计数、淋巴细胞计数、血小板计数、血红蛋白、肝转氨酶、C反应蛋白[CRP]、降钙素原和肌酐）的数据。
我们证明，与健康参与者相比，COVID-19患者血清中IFN-α2、TNF-α、MCP-1/CCL2、IL-6、IL-8、IL-18、IL-33水平升高。在危重症组中观察到CRP水平升高和淋巴细胞计数降低。纵向分析显示，与健康对照相比，危重症患者的IL-6、IL-18和MCP-1血清水平有统计学显著升高。
发现MCP-1、IL-6和IL-18是危重症COVID-19疾病的最佳预测指标，且MCP-1具有最高的预测性能水平。
